Tsukasa Kawahara, PhD

Experimental Pathology

Assistant Professor
Pathology & Laboratory Medicine

Other Appointments/Positions

Research Instructor, Emory University

Pathology Division(s):
Experimental Pathology

Email Address:
tkawaha@emory.edu

Education:

Doctor of Philosophy   The University of Tokushima Graduate School of Medicine, Tokushima, Japan, 2004 -

Master of Science   The University of Tokushima Graduate School of Medicine, Tokushima, Japan, 2001 -

Honors / Awards:

Thesis Prize of the MIKI Scholarship and Foundation, 2004

OKA Scholar Prize, the University of Tokushima School of Medicine, 2003

The Japan Society for the Promotion of Science (JSPS) predoctoral fellowship, 2002-2004

The Japan Scholarship and Foundation, Scholarship for Graduate Students, 2000-2001

The Japan Scholarship and Foundation, Scholarship for University Students, 1998-1999

Selected Publications:

Published and Accepted Research Articles -

Kuwano Y, Tominaga K, Kawahara T, Sasaki H, Takeo K, Nishida K, Masuda K, Kawai T, Teshima-Kondo S, Rokutan K. Tumor necrosis factor alpha activates transcription of the NADPH oxidase organizer 1 (NOXO1) gene and upregulates superoxide production in colon epithelial cells. Free Radic Biol Med. 2008 Dec 15;45(12):1642-52.

Kawahara T*, Lambeth JD. Phosphatidylinositol (4,5)-bisphosphate Modulates Nox5 Localization via an N-Terminal Polybasic Region. Mol. Biol. Cell. 2008 July 9 (*corresponding author)

Sharma, P., Chakraborty,R., Wang, L., Min, B., Tremblay, M., Kawahara, T. Lambeth, JD., Haque, SJ. Redox Regulation of Interleukin-4 Signaling. Immunity. 2008. 29;4:551-564.

Tominaga K, Kawahara T, Sano T, Toida K, Kuwano Y, Sasaki H, Kawai T,Teshima-Kondo S, Rokutan K. Evidence for cancer-associated expression of NADPH oxidase 1 (Nox1)-based oxidase system in the human stomach. Free Radic Biol Med. 2007;43(12):1627-38.

Kawahara T*, Lambeth JD. Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes. BMC Evol Biol. 2007 Sep 27;7:178. (*corresponding author)

Kawahara T, Quinn MT, Lambeth JD. Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymes. BMC Evol Biol. 2007 Jul 6;7:109.

Kuwano Y, Kawahara T, Yamamoto H, Teshima-Kondo S, Tominaga K, Masuda K, Kishi K, Morita K, Rokutan K. Interferon-gamma activates transcription of NADPH oxidase 1 gene and upregulates production of superoxide anion by human large intestinal epithelial cells. Am J Physiol Cell Physiol. 2006;290(2):C433-43

Kawahara T, Ritsick D, Cheng G, Lambeth JD. Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generation. J. Biol. Chem. 2005;280(36):31859-69.

Kawahara T, Kohjima M, Kuwano Y, Mino H, Teshima-Kondo S, Takeya R,Tsunawaki S, Wada A, Sumimoto H, Rokutan K. Helicobacter pylori lipopolysaccharide activates Rac1 and transcription of NADPH oxidase Nox1 and its organizer NOXO1 in guinea pig gastric mucosal cells. Am. J. Physiol. 2005;288(2):C450-7.

Ago T, Kitazono T, Kuroda J, Kumai Y, Kamouchi M, Ooboshi H, Wakisaka M, Kawahara T, Rokutan K, Ibayashi S, Iida M. NAD(P)H oxidases in rat basilar arterial endothelial cells. Stroke 2005;36(5):1040-6.

Kusumoto K, Kawahara T, Kuwano Y, Teshima-Kondo S, Morita K, Kishi K, Rokutan K. Ecabet sodium inhibits Helicobacter pylori lipopolysaccharide-induced activation of NADPH oxidase 1 or apoptosis of guinea pig gastric mucosal cells. Am. J. Physiol. 2005;288(2):G300-7.

Kawahara T, Kuwano Y, Teshima-Kondo S, Takeya R, Sumimoto H, Kishi K, Tsunawaki S, Hirayama T, Rokutan K. Role of nicotinamide adenine dinucleotide phosphate oxidase 1 in oxidative burst response to Toll-like receptor 5 signaling in large intestinal epithelial cells. J. Immunol. 2004;172(5):3051-8.

Chamulitrat W, Stremmel W, Kawahara T, Rokutan K, Fujii H, Wingler K, Schmidt HH, Schmidt R. A constitutive NADPH oxidase-like system containing gp91phox homologs in human keratinocytes. J. Invest. Dermatol. 2004;122(4):1000-9.

Yoshida LS, Nishida S, Shimoyama T, Kawahara T, Kondo-Teshima S, Rokutan K, Kobayashi T, Tsunawaki S. Superoxide generation by Nox1 in guinea pig gastric mucosal cells involves a component with p67(phox)-ability. Biol. Pharm. Bull. 2004;27(2):147-55.

Chamulitrat W, Schmidt R, Tomakidi P, Stremmel W, Chunglok W, Kawahara T, Rokutan K. Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes. Oncogene 2003;22(38):6045-53.

Yoshida L, Nishida S, Shimoyama T, Kawahara T, Rokutan K, Tsunawaki S. Expression of a p67(phox) homolog in Caco-2 cells giving O(2)(-)-reconstituting ability to cytochrome b(558) together with recombinant p47(phox). Biochem. Biophys. Res. Commun. 2002;296(5):1322-8.

Nikawa T, Ikemoto M, Sakai T, Kano M, Kitano T, Kawahara T, Teshima S, Rokutan K, Kishi K. Effects of a soy protein diet on exercise-induced muscle protein catabolism in rats. Nutrition 2002;18(6):490-5.

Kawahara T, Teshima S, Oka A, Sugiyama T, Kishi K, Rokutan K. Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells. Infect. Immu. 2001;69(7):4382-9.

Kawahara T, Teshima S, Kuwano Y, Oka A, Kishi K, Rokutan K. Helicobacter pylori lipopolysaccharide induces apoptosis of cultured guinea pig gastric mucosal cells. Am. J. Physiol. 2001;281(3):G726-34.

Takahashi A, Wada A, Ogushi K, Maeda K, Kawahara T, Mawatari K, Kurazono H, Moss J, Hirayama T, Nakaya Y. Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein. FEBS Lett. 2001;508(3):484-8.

Kawahara T, Kuwano Y, Teshima-Kondo S, Sugiyama T, Kawai T, Nikawa T, Kishi K, Rokutan K. Helicobacter pylori lipopolysaccharide from type I, but not type II strains, stimulates apoptosis of cultured gastric mucosal cells. J Med Invest. 2001, 48;167-74.

Kawahara T, Kuwano Y, Teshima-Kondo S, Kawai T, Nikawa T, Kishi K, Rokutan K. Toll-like receptor 4 regulates gastric pit cell responses to Helicobacter pylori infection. J Med Invest. 2001;48:190-7.

Teshima S, Kutsumi H, Kawahara T, Kishi K, Rokutan K. Regulation of growth and apoptosis of cultured guinea pig gastric mucosal cells by mitogenic oxidase 1. Am. J. Physiol. 2000;279(6):G1169-76.

Rokutan K, Teshima S, Kawai T, Kawahara T, Kusumoro K, Mizushima T, Kishi K. Geranylgeranylacetone stimulates mucin synthesis in cultured guinea pig gastric pit cells by inducing a neuronal nitric oxide synthase. J. Gastroenterol. 2000;35(9):673-81.

Rokutan K, Miyoshi M, Teshima S, Kawai T, Kawahara T, Kishi K. Phenylarsine oxide inhibits heat shock protein 70 induction in cultured guinea pig gastric mucosal cells. Am. J. Physiol. 2000;279(5):C1506-15.

Kawai T, Teshima S, Kusumoto K, Kawahara T, Kondo K, Kishi K, Rokutan K. A non-toxic heat shock protein 70 inducer, geranyl-geranyl-acetone, restores the heat shock response in gastric mucosa of protein-malnourished rats. J Lab Clin Med. 2000;136(2):138-48.

Other Publications -

Rokutan K, Kawahara T, Kuwano Y, Tominaga K, Nishida K, Teshima-Kondo S. Nox enzymes and oxidative stress in the immunopathology of the gastrointestinal tract. Semin Immunopathol. 2008 Jul;30(3):315-27. Review

Lambeth JD, Kawahara T, Diebold B. Regulation of Nox and Duox enzymatic activity and expression. Free Radic Biol Med. 2007 Aug 1;43(3):319-31. Review.

Rokutan K, Kawahara T, Kuwano Y, Tominaga K, Sekiyama A, Teshima-Kondo S. NADPH oxidases in the gastrointestinal tract: a potential role of Nox1 in innate immune response and carcinogenesis. Antioxid Redox Signal. 2006 Sep-Oct;8(9-10):1573-82. Review.