Pathology Faculty Member

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    Yerkes National Primate Research Center
    954 Gatewood Drive
    Atlanta, GA 30329
    404 712-2838 Phone

    For an expert Pathology consultation,
    or to send a specimen, call:

    1-404-712-5947


    Francois J Villinger, PhD

    HIV/SIV Pathogenesis
    Immunology

    Professor
    Pathology & Laboratory Medicine


    Pathology Faculty Photo

    Other Appointments/Positions

    Associate Director Division of Pathology, Yerkes National Primate Research Center


    Pathology Division(s):
    Experimental Pathology


    Email Address:
    fvillin@emory.edu


    Education:

    Federal Swiss Veterinary Degree   Course of study at the Medical School, University of Lausanne (Switzerland), and the Veterinary Medicine School, University of Zurich., 1978 - 1984

    Doctor of Veterinary Medicine   Thesis on the Diagnosis and Epidemiology of Foot-and-Mouth Disease., - 1986

    Post Graduate Training   Post doctoral position at the USDA Plum Island Animal Disease Center in Greenport, NY, in the immunology of African swine fever virus infection., 1987 - 1989

    Post Graduate Training   Research Associate position in the Pathobiology Department at the Yerkes Regional Primate Research Center, Emory University, Atlanta, GA, for the study of immune mechanisms in AIDS using an Old World primate model for AIDS., 1989 - 1991

    Post Graduate Training   Fellowship from the Swiss National Fund for the Advancement of Scientific Research for the project: "Delineation of pathomechanisms in simian AIDS following infection with various retroviruses." (SNF 84AD-030700), 1991 - 1994

      Swiss Federal Maturitat, Type C., 1978 -



    Research Interests:

    - Research interests: Immunology of infectious diseases, AIDS
    Adoptive transfer of autologous CD4+ T cells as a platform for immune reconstitution in simian AIDS. My lab has focused on antibody based technologies for expanding and conditioning purified CD4+ T cells from nonhuman primates for the purpose of reconstituting immune responses and enhance control of in vivo viral replication in the primate AIDS model. Using this preclinical model, we have been able to markedly reduce the chronic simian immunodeficiency viral replication and disease progression in the absence of prolonged chemotherapy. My lab now strives to define the function and characteristics of the expanded cells as well as their fate in vivo.

    A second focus of the lab is its role as a "Resource for nonhuman primate immune reagents" including the creation of a repository for primate cDNA clones, the construction of DNA vectors for the expression of such NHP molecules and the production and testing of monkey recombinant cytokines. The need for this NCRR funded resource was borne from the realization that human cytokines are generally immunogenic when used in monkeys, thus severely limiting the value of these model for immunotherapies. Reagents generated are distributed either freely or at production cost to other PHS funded investigators.



    Honors / Awards:

    SNF fellowship (Swiss National Foundation) in 1992, 1993 and 1994 for the study of Nonhuman primate models of AIDS., 1992-1994

    Selected Publications:

    Published and Accepted Research Articles -

    Kitabwalla M, Villinger F, Akeefe H, Liao Z, Mayne AE, Gargano L, Conner AP, Maltais JA, Kunas G, Hildreth JE, Ansari AA, Bellotti M. 2005. Enhancement of cell mediated immune responses using lipid depleted lentivirus as immunogen: a novel approach for inducing recognition of new viral epitopes. Vaccine 23:4666-77.


    Mueller YM, Petrovas C, Bojczuk PM, Dimitriou ID, Beer B, Silvera P, Villinger F, Cairns JS, Gracely EJ, Lewis MG, Katsikis PD. 2005. Interleukin-15 Increases Effector Memory CD8+ T Cells and NK Cells in Simian Immunodeficiency Virus-Infected Macaques. J Virol 79:4877-85.


    Switzer WM, Salemi M, Shanmugam V, Gao F, Cong ME, Kuiken C, Bhullar V, Beer BE, Vallet D, Gautier-Hion A, Tooze Z, Villinger F, Holmes EC, Heneine W. 2005. Ancient co-speciation of simian foamy viruses and primates. Nature 434:376-80.


    Y Sui, S Li, I Adany, D Pinson, Z Li, F Villinger, O Narayan, S Buch. 2005. Simian Human Immunodeficiency Virus-Associated Pneumonia Correlates with Increased Expression of MCP-1, CXCL10, and Viral RNA in the Lungs of Rhesus Macaques. Am J Pathol 166:355-65.


    Amara RR, Ibegbu C, Villinger F, Montefiori DC, Sharma S, Nigam P, Xu Y, McClure HM, Robinson HL. 2005. Studies using a viral challenge and CD8 T cell depletions on the roles of cellular and humoral immunity in the control of an SHIV-89.6P challenge in DNA/MVA-vaccinated macaques. Vaccine 343:246-55


    NK Dhillon, Y Sui, R Potula, S Dhillon, I Adany, Z Li, F Villinger, D Pinson, O Narayan, S Buch. 2005. Inhibition of pathogenic SHIV replication in macaques treated with antisense DNA of interleukin-4. Blood 105:3094-9.


    Amara RR, Ibegbu C, Villinger F, Montefiori DC, Sharma S, Nigam P, Xu Y, McClure HM, Robinson HL. 2005. Studies using a viral challenge and CD8 T cell depletions on the roles of cellular and humoral immunity in the control of an SHIV-89.6P challenge in DNA/MVA-vaccinated macaques. Vaccine 343:246-55


    F Villinger, R Miller, K Mori, AE Mayne, P Bostik, JB Sundstrom, C Sugimoto and AA Ansari. 2004. IL-15 is superior to IL-2 in the generation of long-lived antigen specific memory CD4 and CD8 T cells in rhesus macaques. Vaccine 22:3510-21


    P Bostik, M Watkins, F Villinger and AA Ansari. 2004. Genetic analysis of cytokine promoters in nonhuman primates: Implication for Th1/Th2 profile characteristics and SIV disease pathogenesis. Clin Dev Immunol 11:35-44


    JB Sundstrom, DM Little, F Villinger, JE Ellis and AA Ansari. 2004. Signalling though toll-like receptors triggers HIV-1 replication in latently infected Mast cells. J Immunol 172:4391-4401.


    SA Safley, F Villinger, EH Jackson, C Tucker-Burden, C Cohen and CJ Weber. 2004. Interleukin-6 (IL-6) secretion by human parathyroids. Cell Immunol 136:145-156.


    AA Ansari, AE Mayne, N Onlamoon, K Pattanapanyasat, K Mori and F Villinger. 2004. Use of recombinant cytokines for optimized induction of antiviral immunity against SIV in the nonhuman primate model of human AIDS. Immunologic Research 29:1-17


    JB Sundstrom, H Mao, R Santoianni, TT Huynh, DL Little, F Villinger, AE Mayne, E Hao and AA Ansari. 2004. Magnetic resonance imaging (MRI) of activated proliferating rhesus macaque T cells labeled with superparamagnetic monocrystalline iron oxide nanoparticles (MION). J AIDS 35:9-21.


    P Bostik, GL Dodd, F Villinger, AE Mayne and AA Ansari. 2004. Dysregulation of the polo-like kinase pathway in CD4+ T cells is characteristic of pathogenic simian immunodeficiency virus infection. J Virol. 78:1464-72.


    S Czub, M Czub, E Koutsilieri, S Sopper, F Villinger, JG Muller, C Stahl-Hennig, P Riederer, V Ter Meulen and G Gosztonyi. 2004. Modulation of simian immunodeficiency virus neuropathology by dopaminergic drugs. Acta Neuropathol 107:216-26.


    Sundstrom JB, Jollow KC, Braud V, Villinger F, McMichael AJ, Lawrencec EC, Ades EW, Ansari AA. 2003. Magnitude of alloresponses to MHC class I/II expressing human cardiac myocytes is limited by their intrinsic ability to process and present antigenic peptides. Clin Dev Immunol.10:213-26.


    SL Buge, HL Ma, RR Amara, LS Wyatt, PL Earl, F Villinger, DC Montefiori, SI Staprans, Y Xu, E Carter, SP O'Neil, JG Herndon, E Hill, B Moss, HL Robinson and JM McNicholl. 2003. Gp120-Alum Boosting of a Gag-Pol-Env DNA/MVA AIDS Vaccine: Poorer Control of a Pathogenic Viral Challenge. AIDS Hum Retrov Res 19:891-900.


    F Villinger, AE Mayne, P Bostik, K Mori, PE Jensen, R ahmed and AA Ansari. 2003. Evidence for antibody mediated enhancement of SIV Gag antigen processing and cross presentation in SIV infected rhesus macaques. J Virol 77:10-24.


    Sui Y, Potula R, Pinson D, Adany I, Li Z, Day J, Buch E, Segebrecht J, Villinger F, Liu Z, Huang M, Narayan O, Buch S. Microarray analysis of cytokine and chemokine genes in the brains of macaques with SHIV-encephalitis. 2003. J Med Primatol 32:229-239.


    E O'Neill, V Bostik, DC Montefiori, E Kraiselburd and F Villinger. 2003. IL-12/GM-CSF co-administration in an SIV DNA prime/protein boost protocol enhances Gag specific T cells but not virus-specific neutralizing antibodies in rhesus macaques. AIDS Hum Retrov Res 19:883-890.


    Cytokines as clinical adjuvants: How far are we? Villinger. 2003 In: Expert Rev of Vaccines 2:317-326.


    P Lena, F Villinger, L Giavedoni, CJ Miller, G Rhodes and P Luciw. 2002. Co-immunization of rhesus macaques with plasmid vectors expressing IFN-gamma, GM-CSF, and SIV antigens enhances anti-viral humoral immunity but does not affect viremia after challenge with highly pathogenic virus. Vaccine 20, Suppl 4:A69-79.


    Y Tang, F Villinger, SI Staprans, RR Amara, JM Smith, JG Herndon and HL Robinson. 2002. Slowly declining levels of viral RNA and DNA in DNA/recombinant modified vaccinia virus Ankara-vaccinated macaques with controlled simian-human immunodeficiency virus SHIV-89.6P challenges. J Virol 76:10147-10154.


    RR Amara, F Villinger, JD Altman, S Lydy, SP O'Neil, SI Staprans, DC Montefiori, Y Xu, JG Herndon, LS Wyatt, M Candido, NL Kozyr, PL Earl, JM Smith, HL Ma, B Grimm, ML Hulsey, HM McClure, JM McNichols, B Moss and HL Robinson. 2002. Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine. Vaccine 20:1949-1955.


    PH Van der Meide, Villinger F, Ansari AA, Groenestein RJ, de Labie MC, van den Hout YJ, Koornstra WH, Bogers WM, Heeney JL. 2002. Stimulation of both humoral and cellular immune responses to HIV-1 gp120 by interleukin-12 in Rhesus macaques. Vaccine 20:2296-2302.


    RR Amara, F Villinger, SI Staprans, JD Altman, DC Montefiori, NL Kozyr, Y Xu, LS Wyatt, PL Earl, JG Herndon, HM McClure, B Moss and HL Robinson. 2002. Different patterns of immune responses but similar control of a simian-human immunodeficiency virus 89.6P mucosal challenge by modified Vaccinia virus Ankara (MVA) and DNA/MVA vaccines. J Virol 76:7625-7631.


    B Makitalo, M Andersson, I Arestrom, K Karlen, F Villinger, A Ansari, S Paulie, R Thorstensson, N Ahlborg. 2002. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods 270:85-97.


    SJ Buch, F Villinger, D Pinson, Hou Y, Adany I, Z Li, R Dalal, R Raghavan, A Kumar and O Narayan. 2002. Innate differences between simian-human immunodeficiency virus (SHIV)KU-2 infected rhesus and pig-tailed macaques to development of neurological disease. Virology 295:54-62.


    E O'Neill, I Martinez, F Villinger, M Rivera, S Gascot, C Colon, T Arana, M Sidhu, R Stout, DC Montefiori, M Martinez, AA Ansari, ZR Israel and E Kraiselburd. 2002. Protection by SIV VLP DNA prime/protein boost following mucosal SIV challenge is markedly enhanced by IL-12/GM-CSF co-administration. J Med Primatol 31:217-227.


    A Hicks, R Potula, F Villinger, D Pinson, I Adany, Z Li, Y Hou, C Long, N Mueller, A Kumar, E Major, O Narayan and S Buch. 2002. Neuropathogenesis of lentiviral infection in macaques: Role of CXCR4 and CCR5 viruses and IL-4 in enhancing monocyte chemoattractant protein-1 production in macrophages. Am J Pathol. 161:813-822.


    AA Ansari, AE Mayne, JB Sundstrom, P Bostik, B Grimm, JD Altman and F Villinger. 2002. rMamu-IL12 administration during acute SIV infection leads to decreased viral loads associated with prolonged survival in sivmac251 infected rhesus macaques. J Virol 76:1731-1743.


    S Kumar, F Villinger, M Oakley, JC Aguiar, TR Jones, RC Hedstrom, K Gowda, J Chute, A Stowers, DC Kaslow, EK Thomas, J Tine, D Klinman SL Hoffman and WW Weiss. 2002. A DNA vaccine encoding the 42KD C-terminus of merozoite surface protein 1 of Plasmodium falciparum induces antibody, interferon-g and cytotoxic T cell response in rhesus monkeys: Immunostimulatory effects of granulocyte macrophage-colony stimulating factor. Immunol Lett 81:13-24.


    F Villinger, GT Brice, AE Mayne, P Bostik, K Mori, CH June and AA Ansari. 2002. Extended control of SIV replication following adoptive transfer of pre-infection lymphoid cells in SIV infected rhesus macaques. Blood 99:590-599.


    RR Amara, JM Smith, SI Staprans, DC Montefiori, F Villinger, JD Altman, SP O'Neil, NL Kozyr, Y Xu, LS Wyatt, PL Earl, JG Herndon, JM McNicholl, HM McClure, B Moss and HL Robinson. 2002. Critical role for Env as well as Gag-Pol in control of a simian-human immunodeficiency virus 89.6P challenge by a DNA prime/recombinant modified Vaccinia virus Ankara vaccine. J Virol 76:6138-6146.


    P Bostik, P Wu, GL Dodd, F Villinger, AE Mayne, V Bostik, BD Grimm, D Robinson, HJ Kung and AA Ansari. 2001. Identification of protein kinases dysregulated in CD4(+) T cells in pathogenic versus apathogenic simian immunodeficiency virus infection. J Virol 75:11298-11306.


    70. RR Amara, F Villinger, JD Altman, SL Lydy, SP O'Neil, SI Staprans, DC Montefiori, Y Xu, JG Herndon, LS Wyatt, MA Candido, NL Kozyr, PL Earl, JM Smith HL Ma, BD Grimm, ML Hulsey, HM McClure, JM McNicholl, B Moss and HL Robinson. 2001. Control of a mucosal challenge and prevention of AIDS in rhesus macaques by a multiprotein DNA/MVA vaccine. Science 292:69-74.


    GH Giambartolomei, BL Lasater, F Villinger and VA Dennis. 2001. Diminished expression of the costimulatory ligand CD80 (B7.1) gene correlates with antigen specific cellular unresponsiveness in rhesus macaques with lymphatic filariasis. Acta Tropica 78:67-7185.


    S Buch, D Pinson, CL King, R Raghavan, Y Hou, Z Li, I Adany, A Hicks, F Villinger A Kumar and O Narayan. 2001.Inhibitory and enhancing effect of IFN-g and IL-4 on SHIVKU replication in rhesus macaque macrophages: correlation between Th2 cytokines and productive infection in tissue macrophages during late stage infection. Cytokines 13:295-304.


    F Villinger, P Bostik, AE Mayne, CP Genain, CL King, WR Weiss and AA Ansari. 2001 Molecular characterization of cell-associated-molecules (CAM) and co-stimulatory-molecules (CSM) from nonhuman primates. Immunogenetics 15:315-328.


    P Bostik, AE Mayne, F Villinger, K Greenberg, JD Powell and AA Ansari. 2001. Relative resistance in the development of T cell anergy in CD4+ T cells from SIV disease resistant sooty mangabeys. J Immunol. 166:506-516.


    F Villinger, T Rowe, BS Parekh, T Green, AE Mayne, B Grimm, HM McClure, A Lackner, AA Ansari and TM Folks. 2001. Chronic immune stimulation accelerates SIV induced disease progression. J Med Primatol 30:254-259.


    KL Hutchinson, F Villinger, ME Miranda, TG Ksiazek, CJ Peters and PE Rollin. 2001 Multiplex analysis of cytokines in the sera of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol 65:561-565.


    AA Ansari, P Bostik, AE Mayne and F Villinger. Failure to expand influenza and tetanus toxoid memory T cells in vitro correlates with disease course in SIV infected rhesus macaques. Cell Immunol 210:125-42, 2001.


    F Villinger, NF Chikkala, SS Brar, SZ Bucur, ME Lee, DA Lackey III, JA Adams, P Bostik, AE Mayne, F Novembre, AA Ansari and CD Hillyer. 2000. Late decrease in in vivo IL-12 responsiveness in macaques after SIVmac239 infection. AIDS Hum Retrov 16:751-763.


    T Lehner, Y Wang, M Cranage, L Tao, E Mitchell, C Bravery, C Doyle, K Pratt, G Hall, M Dennis, F Villinger and L Bergmeier. 2000. Upregulation of b chemokines and downmodulation of CCR5 co-receptors inhibit simian immunodeficiency transmission in nonhuman primates. Immunology 99:569-577.


    P Bostik, J Winter, TG Ksiazek, PE Rollin, F Villinger, SR Zaki, CJ Peters and AA Ansari. 2000. Sin Nombre Virus (SNV) Ig Isotype Antibody Response during Acute and Convalescent Phases of Hantavirus Pulmonary Syndrome. Emerging Inf Dis 6.


    T Lehner, E Mitchell, L Bergmeier, M Singh, R Spallek, M Cranage, G Hall, M Dennis, F Villinger and Y Wang. 2000. The role of gd T cells in generating antiviral factors and b-chemokines in protection against mucosal simian immunodeficiency virus infection. Eur J Immunol 30.


    J Arredondo, FX Lu, F Villinger, MB McChesney, J McGhee and CJ Miller. 2000. Antigen-dependent cytokine mRNA expression by individual Rhesus macaque T helper cells by flow cytometry. Cell Immunol 201: 94-108.


    P Bostik, GT Brice, KP Greenberg, AE Mayne, F Villinger, MG Lewis and AA Ansari. 2000. Inverse correlation of telomerase activity/proliferation of CD4+ T lymphocytes and disease progression in simian immunodeficiency virus-infected nonhuman primate. J Acquir Immunodef Syndr 24:89-99.


    SZ Bucur, TW Gillespie, ME Lee, JW Adams, RA Bray, F Villinger, AA Ansari and CD Hillyer. 2000. Hematopoietic response to lineage-non-specific (IL-3) and lineage-specific (rhG-CSF, rhEPO, rhTPO) cytokine administration in SIV-infected macaques is related to stage of infection J Med Primatol 29:47-56.


    K Mori, Yasutomi Y, Sawada S, Villinger F, Sugama K, Rosenwith B, Heeney JL, Uberla K, Yamazaki S, Ansari AA, Rubsamen-Waigmann H. 2000. Suppression of acute viremia by short-term postexposure prophylaxis of simian/human immunodeficiency virus SHIV-RT-infected monkeys with a novel reverse transcriptase inhibitor (GW420867) allows for development of potent antiviral immune responses resulting in efficient containment of infection. J Virol 74:5747-5753.


    F Villinger, WM Switzer, BS Parekh, RA Otten, D Adams, V Shanmugam, P Bostik, AE Mayne, NF Chikkala, HM McClure, F Novembre, Q Yao, W Heneine, TM Folks and AA Ansari. 2000. Induction of long-term protective effects against heterologous challenge in SIVhu infected macaques. Virol 278:194-206.


    Y Zhu, K Koo, JD Bradshaw, WF Sutton, LR Kuller, R Bucala, D Anderson, SP Mossman, F Villinger and NL Haigwood. 2000. Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses. J Med Primatol 29:182-192.


    GT Brice, AE Mayne, F Villinger and AA Ansari. 2000. A novel role for tumor necrosis factor-a in regulating susceptibility of activated CD4+ T cells from human and nonhuman primates for distinct coreceptor using lentiviruses. J AIDS 24:10-22.


    F Villinger, PE Rollin, SS Brar, NF Chikkala, J Winter, JB Sundstrom, SR Zaki, R Swanepoel, AA Ansari and CJ Peters. 1999. Markedly elevated levels of interferon (IFN)-g, IFN alpha, interleukin (IL)-2, IL-10 and tumor necrosis factor-a associated with fatal Ebola virus infection. J Inf Dis 178:S188-S191


    GT Brice, AE Mayne, K Leighton, F Villinger, JS Allan and AA Ansari. 1999. Studies of CD40L expression by lymphoid cells from experimentally and naturally SIV-infected nonhuman primate species. J Med Primatol 28:49-56.


    F Villinger, GT Brice, Ann Mayne, P Bostik and AA Ansari. 1999. Control mechanisms of virus replication in naturally SIVsmm infected mangabeys and experimentally infected macaques. Immunology Letters 66:37-46.


    Z Smit-McBride, J Mattapallil, F Villinger, A A Ansari and S Dandekar. 1998. Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques. J Med Primatol 27:129-140.


    SZ Bucur, DA Lackey III, JW Adams, ME Lee, F Villinger, A Mayne, RA Bray, EF Winton, F Novembre, EA Strobert, J De Rosayro, PJ Dailey, AA Ansari and CD Hillyer. 1998. Hematologic and virologic effects of lineage-specific and non-lineage-specific recombinant human (rh) and rhesus (rr) cytokines in a cohort of SIVmac239-infected macaques. AIDS Res Hum Retr 14:651-660.


    V Courgnaud, W Saurin, F Villinger and P Sonigo. 1998. Evolution differences of simian immunodeficiency virus in a natural host and a new host. Virol 247:41-50.


    S Duque, S Montenegro-James, M Arevalo-Herreras, AD Praba, F Villinger, S Herrera and MA James. 1998. Expression of cytokine genes in Aotus monkeys immunized with synthetic and recombinant Plasmodium vivax and P. falciparum antigens. Ann Trop Med Parasitol 92:553-559.


    GT Brice, JL Riley, F Villinger, A Mayne, CD Hillyer, CH June and AA Ansari. 1998. Development of an animal for autotransfusion therapy: In vitro characterization and analysis of anti-CD3/CD28 expanded cells. J AIDS Hum Retrov 19:210-220.


    P Bostik, F Villinger, GT Brice, NF Chikkala, SS Brar, WW Cruikshank, JW Adams, CD Hillyer and AA Ansari. 1998. Expression and in vitro evaluation of rhesus macaque wildtype and modified chemokines. J Med Primatol 27:113-120.


    ME Lee, JW Adams, F Villinger, S Brar, DA Lackey, W Cruikshank, AA Ansari and CD Hillyer. 1998 . Molecular cloning and expression of rhesus macaque interleukin-16 and its inhibition of simian immunodeficiency virus replication. AIDS Res Hum Retrov 14:1323-1328.


    GH Giambartolomei, B Lasater, F Villinger and VA Dennis. 1998. Diminished production of T helper 1 cytokines and lack of induction of IL-2R+ T cells correlate with T-cell unresponsiveness in rhesus monkeys chronically infected with Brugia malayi. Exp. Parasitology 90:77-85.


    P Bostik, F Villinger, TM Folks and AA Ansari. 1997. Pre-infection CD4+:CD8+ ratio and HIV infection. Letter to the Editor. Immunol Today 18:555.


    YJ Rosenberg, A Cafaro, T Brennan, JG Greenhouse, F Villinger, AA Ansari, C Brown, K McKinnon, S Beliah, J Yalley-Ogunro, WR Elkin, S Gartner and MG Lewis. 1997. Virus-induced cytokines regulate circulating lymphocyte levels during primary SIV infections. Int Immunol 9:703-712.


    F Villinger, SS Brar, GT Brice, NF Chikkala, FJ Novembre, AE Mayne, S Bucur, CD Hillyer and AA Ansari. 1997. Immune and hematopoietic parameters in HIV-1 infected Chimpanzees during clinical progression towards AIDS. J Med Primatol 26:11-18.


    TM Folks, F Villinger and AA Ansari. 1997. Immune activation in lentivirus infection: From Charybdis into Scylla? Research in: HIV and Cytokines, M Guenounou (Ed), Les Editions Inserm, 7-13.


    TM Folks, T Rowe, F Villinger, B Parekh, A Mayne, D Anderson, H McClure and AA Ansari. 1997. Immune stimulation enhances progression of SIV disease in rhesus macaques. J Med Primatol 26:181-189.


    SL Hoffman, JM Crutcher, SK Puri, AA Ansari, F Villinger, ED Franke, PP Singh, F Finkelman, MK Gately, GP Dutta and M Sedegah. 1997. Sterile protection of monkeys against malaria after administration of interleukin-12. Nature Med 3:80-83.


    GT Brice, F Villinger, A Mayne, JB Sundstrom and AA Ansari. 1996. Detection of intracellular signal transduction molecules in PBMC from rhesus macaques and sooty mangabeys. J Med Primatol 25:210-217.


    O Benveniste, Vaslin B, F Villinger, R Le Grand, AA Ansari and D Dormont. 1996. Cytokine mRNA levels in unmanipulated (ex vivo) and in vitro stimulated monkey PBMCs using a semiquantitative RT-PCR and high sensitivity fluorescence-based detection strategy. Cytokine 8:32-41.


    F Villinger, TM Folks, S Lauro, JD Powell JB Sundstrom, A Mayne and AA Ansari. 1996. Immunological and virological studies of natural SIV infection of disease resistant nonhuman primates. Immunology Letters 51:59-68.


    CP Genain, K Abel, N Belmar, F Villinger, DP Rosenberg, C Linington, CS Raine and SL Hauser. 1996. Late complications of immune deviation therapy in a nonhuman primate. Science 274:2054-2057.


    AA Ansari, JB Sundstrom, K Kanter, A Mayne, F Villinger, MB Gravanis and A Herskowitz. 1995. Cellular and molecular mechanisms of human cardiac myocyte injury after transplantation. J. Heart Lung Transpl. 14:102-112.


    AA Ansari, JB Sundstrom, K Kanter, A Mayne, F Villinger, MB Gravanis and A Herskowitz. 1995. Cellular and molecular mechanisms of human cardiac myocyte injury after transplantation. J. Heart Lung Transpl. 14:102-112.


    AA Ansari, A Mayne, JB Sundstrom, MB Gravanis, K Kanter, KW Sell, F Villinger, CO Siu and A Herskowitz. 1995. Frequency of hypoxanthine guanine phosphoribsyltransferase (hprt-) T cells in the peripheral blood of cardiac transplant recipients: A noninvasive technique for the diagnosis of allograft rejection. Circulation 92:862-874.


    TF Neal, HK Holland, CM Baum, F Villinger, AA Ansari, R Saral, JR Wingard and WH Fleming. 1995. CD34+ progenitors from asymptomatic patients are not a major reservoir for HIV-1. Blood 86:1749-1756.


    F Villinger, S Brar, A Mayne, N Chikkala and AA Ansari. 1995. Comparative analysis of cytokine genes from nonhuman primates. J. Immunol. 155:3945-3954.


    AA Ansari, N Neckelmann, F Villinger, P Leung, DJ Danner, SS Brar, S Zhao, MB Gravanis, A Mayne, ME Gershwin and A Herskowitz. 1994. Epitope mapping of the branched chain alpha-ketoacid dehydrogenase-E2 (BCKD-E2) protein that reacts with sera from patients with idiopathic dilated cardiomyopathy. J. Immunol. 153:4754-4765.


    T Jehuda-Cohen, JD Powell, F Villinger, A Mayne, KW Sell and A. Ahmed-Ansari. 1994. Evidence for for antigen-specific memory B cells in seronegative nonhuman primates naturally infected with the simian immunodeficiency virus. J. AIDS. 7:539-550


    AA Ansari, A Mayne, D Hunt, JB Sundstrom and F Villinger. 1994. TH1/TH2 subset analysis. I Establishment of criteria for subset identification in PBMC samples from nonhuman primates. Am. J. Primatol. 23:102-107.


    M Knuchel, DP Bednarik, N Chikkala, F Villinger, TM Folks and AA Ansari. 1994. Development of a novel quantitative assay for the measurement of chloramphenicol acetyl transferase (CAT) mRNA. J. Virol. Meth. 48:325-338.


    AA Ansari, Y-C Wang, K Kanter, F Villinger, A Mayne, KW Sell and A Herskowitz. 1993. Host T-cell primary allosensitization to MHC class I- and II- expressing human cardiac myocytes requires the presence of a second signal. Hum. Immunol. 37:108-118.


    WG Rice, CA Schaefer, B Harten, F Villinger, TL South, MF Summers, LE Henderson, JW Bess, LO Arthur, JS McDougal, SL Orloff, J Mendeleyev and E Kun. 1993. Zinc ejection from HIV-1 zinc fingers and inhibition of infectivity in human lymphocytes by novel aromatic C-nitroso compounds. Nature 361:473-475


    WG Rice, CA Schaefer, L Graham, M Bu, JS McDougal, SL Orloff, F Villinger, M Young, S Oroszlan, MR Fesen, J Mendeleyev and E Kun. 1993. The site of antiviral action of 3-nitrosobenzamide on the infectivity process of human immunodeficiency virus in human lymphocytes. Proc. Natl. Acad. Sci. USA 90:9721-9724.


    F Villinger, D Hunt, A Mayne, M Vuchetich, H Findley and AA Ansari. 1993. Standardization of functional, EIA, and molecular assays for cytokines in rhesus macaques and mangabeys. Cytokine 5:469-479.


    CD Hillyer, DA Lackey III, F Villinger, EF Winton, HM McClure and AA Ansari. 1993. CD34+ and CFU-GM progenitors are significantly decreased in SIVsmm9 infected rhesus macaques with minimal evidence of direct viral infection by polymerase chain reaction. Am. J. Hematol. 43:274-278.


    JD Powell, DP Bednarik, TM Folks, T Jehuda-Cohen, F Villinger, KW Sell and AA Ansari. 1993. Inhibition of cellular activation of retroviral replication by CD8+ T cells derived from nonhuman primates. Clin. Exp. Immunol. 91:473-481.


    DL Rudolph, JA Yee, J Mone, SK Foung, JJ Lipka, GR Reyes, K Hadlock, L Chan, F Villinger,MD Lairmore, S Sinha and RB Lal. 1992. Serologic confirmation of simian T-lymphotropic virus type 1 infection by using immunoassays developed for human T-lymphotropic virus antibody detection. J. Clin. Microbiol. 30(4):858-862.


    BK De, MD Lairmore, K Griffis, LJ Williams, F Villinger, TC Quinn, C Brown, Nzilambi, M Sugimoto, S Araki and TM Folks. 1991. Comparative analysis of nucleotide sequences of the partial envelope gene (5'domain) among human T lymphotropic virus type I (HTLV-I) isolates. Virology 182:413-419.


    HM McClure, DC Anderson, P Fultz, A Ahmed-Ansari, T Jehuda-Cohen, F Villinger, SA Klumpp, W Switzer, E Lockwood, A Brodie. 1991. Maternal Transmission of SIVsmm in rhesus macaques. J. Med. Primatol. 20:182-187.


    T Jehuda-Cohen, JD Powell, KW Sell, F Villinger, E Lockwood, HM McClure, and A Ahmed-Ansari. 1991. Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates. J. Inf. Dis. 163:1223-1228.


    T Jehuda-Cohen, F Villinger, JD Powell, HM McClure, KW Sell, A. Ahmed-Ansari. 1991 Presence of SIV antibodies in the sera of infants born to SIV-seronegative monkeys. J. AIDS 4:204-206.


    JD Powell, DP Bednarik, T Jehuda-Cohen, F Villinger, TM Folks and AA Ansari. 1991. Regulation of immune activation/retroviral replica-tion by CD8+ T cells. Ann. N.Y. Acad. Sci. 363:360-362.


    F Villinger, JD Powell, T Jehuda-Cohen, N Neckelmann, BK De, TM Folks, HM McClure, and A. Ahmed-Ansari. 1991. Detection of occult simian immunodeficiency virus SIVsmm infection in asymptomatic seronegative nonhuman primates and evidence for variation in SIV gag sequence between in vivo- and in vitro-propagated virus. J. Virol. 65(4):1855-1862.


    JD Powell, F Villinger, T Jehuda-Cohen, M Vuchetich, HM McClure, KW Sell, A Ahmed-Ansari. 1990. Identification of SIV/SMM viral proteins that induce T cell response in experimentally infected rhesus macaques and naturally infected sooty mangabeys by the cellular Western blot assay. Preliminary results. J. Med. Primatol. 19:227-238.


    JD Powell, T Jehuda-Cohen, F Villinger, HM McClure, KW Sell, A Ahmed-Ansari. 1990. Inhibition of SIV/SMM replication in vitro by CD8+ cells from SIV/SMM infected seropositive clinically asymptomatic sooty mangabeys. J. Med. Primatol. 19:239-249.


    T Jehuda-Cohen, J Powell, F Villinger, DC Anderson, HM McClure, KW Sell, A Ahmed-Ansari. 1990. Comparison of SIV/SMM replication in CD4+ T cell and monocyte/ macrophage cultures from rhesus macaques and sooty mangabeys. J. Med. Primatol. 19:251-267.


    T Jehuda-Cohen, J Powell, F Villinger, DC Anderson, HM McClure, KW Sell, A Ahmed-Ansari. 1990. Comparison of SIV/SMM replication in CD4+ T cell and monocyte/ macrophage cultures from rhesus macaques and sooty mangabeys. J. Med. Primatol. 19:251-267.


    T Jehuda-Cohen, BA Slade, JD Powell, F Villinger, B De, TM Folks, HM McClure, KW Sell, and A. Ahmed-Ansari. 1990. Polyclonal B cell activation reveals antibodies against human immunodeficiency virus type 1 (HIV-1) in HIV-1-seronegative individuals. Proc. Nat. Acad. Sci. 87:3972-3976.


    EV Genovesi, F Villinger, DJ Gerstner, TC Whyard and RC Knudsen. 1990 Swine monocytes/macrophages retain their susceptibility for infection with African swine fever virus when maintained in vitro in the presence of murine macrophage colony-stimulating factor (CSF-1). Vet. Microbiol. 25 153-176.


    HK Mueller, F Villinger, C. Griot, M Ackermann, L Bruckner and U Kihm. 1989. Vaccination against foot- and mouth disease in Switzerland. I. Potency testing and herd immunit Schweiz. Arch. Tierhelik. 131 379-386.


    F Villinger, EV Genovesi, TC Whyard, DJ Gerstner and RC Knudsen. 1989. In vitro inhibition of African swine fever virus growth in cultured swine macrophages by phosphonoacetic acid and phosphonoformic acid. Arch. of Virol. 115;163-184.


    F Villinger, HK Mueller, M Ackermann, L Bruckner and U Kihm. 1989 Antibodies to Foot and Mouth Disease (FMD) virus infection associated (VIA) antigen: Use of a bioengineered VIA protein as antigen in an ELISA. Vet. Microbiol. 20 235-246.


    Book Chapters -

    G Voss and F Villinger. 2000 Adjuvanted vaccine strategies and live vector approaches for the prevention of AIDS. In: AIDS 14: Suppl 3


    Benveniste, M Martin, F Villinger and D Dormont. 1998. Techniques for quantification of Cytokine mRNAs. In: Cytokines and Molecular Therapy 4:207-214.


    TM Folks, F Villinger and AA Ansari. 1997. Immune activation in lentivirus infection: From Charybdis into Scylla? Research in: HIV and Cytokines, M Guenounou (Ed), Les Editions Inserm, 7-13.


    JD Powell, HM McClure, F Villinger and AA Ansari. 1990 Immunologie des primatesIn: Immunologie Animale Profs Pastoret, Govaerts et Bazin (Eds) Flammarion.


    Other Publications -

    JB Sundstrom, DM Little, F Villinger, JE Ellis and AA Ansari. Stimulation through Toll-Like receptors or IgE cross-linking induces HIV replication in resting latently infected human mast cells: Role for mast cells as a viral reservoir in persistent HIV-1 infection. Keystone Symposia on HIV Vaccine Development; Progress and prospects, April 14-18 2004, Whistler Resort, Canada.


    A Ansari, F Villinger, JEK Hildreth, M Bellotti, JB Maltais, H Akeefe, T Perlman, A Conner, G Kunas and M Kitabwalla. A Prime-Boost Immunization Strategy Using Delipidated SIV Gives Rise to a Broader CD4+ and CD8+ T-Cell Responses in Mice than AT-2 Treated or Live Virus-A Novel Therapeutic Vaccine Approach for HIV Infection. Keystone Symposia on HIV Vaccine Development; Progress and prospects, April 14-18 2004, Whistler Resort, Canada.


    YM Mueller, C Petrovas, P Bojczuk, ID Dimitriou, B Beer, P Silvera, F Villinger, JS Cairns, MG Lewis and PD Katsikis. Selective CD8+ T cell expansion in SIV-infected primates treated with interleukin 15 (IL-15). Keystone Symposia on HIV Vaccine Development; Progress and prospects, April 14-18 2004, Whistler Resort, Canada.


    YM Mueller, C Petrovas, P Bojczuk, ID Dimitriou, B Beer, P Silvera, F Villinger, JS Cairns, MG Lewis and PD Katsikis. In vivo treatment with IL-15 increases CD8+ T cell number in SIV-infected primates. Experimental Biology 2004, April 17-21, Washington, DC.


    C Petrovas, YM Mueller, P Bojczuk, ID Dimitriou, B Beer, P Silvera, F Villinger, JS Cairns, MG Lewis and PD Katsikis. IL-15 Treatment of SIV-infected Non-human Primates. Eleventh Conference on Retrovirus and Opportunistic Infections, San Francisco, Feb 8-11, 2004.


    A Ansari , F Villinger, JEK Hildreth, A Mayne and JB Maltais.Use of solvent treated SIV to induce/augment SIV specific CD4+ and CD8+ T cell memory responses in mice. A model for a new therapeutic vaccination strategy against HIV infection. Eleventh Conference on Retrovirus and Opportunistic Infections, San Francisco, Feb 8-11, 2004.


    F Villinger, R Miller, AE Mayne, P Bostik, JB Sundstrom and AA Ansari. IL-2 failed to boost CD8 responses in rhesus macaques while IL-15 boosted antigen specific memory responses. Abstract 491, The 2nd IAS Conference on HIV Pathogenesis and Treatment, Paris, France, July 13-16, 2003.


    P Lena, MB McChesney, F Villinger, J Smith, PA Luciw, M Marthas, CJ Miller, JV Torres, RW Malone, MB Gardner and GH Rhodes. Controle de l'infection par SIVmac251 et de la maladie apr¿s vaccination par ADN. Ve Journees francophones Virologies, Paris FR, April 10-11, 2002.


    Ansari AA, AE Mayne, K Mori, P Bostik, R Ahmed, PE Jensen and F Villinger. CD4+ T cell mediated and antibody induced enhancement of SIVgag antigen and cross presentation in SIV infected rhesus macaques. Annual Symposium on Nonhuman Primate Models of AIDS, Monterey CA, Sept 8-11, 2002.


    F Villinger, R Miller, AE Mayne, V Bostik, P Bostik, B Grimm, JB Sundstrom and AA Ansari. IL-15 is superior to IL-2 in the generation of long-lived antigen specific memory CD4+ and CD8+ T cells in rhesus macaques. Abstract #, 20th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey CA, Sept 8-11, 2002.


    F Villinger, V Bostik, AE Mayne, P Bostik, D Adams, CN Kim, RA Otten, W Heneine, WM Switzer, F Novembre, HM McClure, TM Folks and AA Ansari. SIVhu is apathogenic for newborn rhesus macaques but protection against heterologous SHIV89.6p challenge requires detectable perinatal replication of SIVhu. Abstract #, 20th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey CA, Sept 8-11, 2002.


    Amara RA, F Villinger, J Altman, S Staprans, N Kozyr, J McNicholl and H Robinson. MVA compared to DNA/MVA-vaccine; slower but similar long-term control of SHIV89.6p challenge. Abstract #103, Keystone Symposium on Experimental AIDS Vaccines, Keystone CO, Apr 5-12, 2002.


    F Villinger, R Miller, AE Mayne, V Bostik, B Grimm, P Bostik and AA Ansari. IL-15 is superior to IL-2 as an adjuvant for durable T cell memory in rhesus macaques. Abstract #435, Keystone Symposium on Experimental AIDS Vaccines, Keystone CO, Apr 5-12, 2002.


    P Bostik, K Greenberg, G Dodd, P Wu, F Villinger and AA Ansari. Distinct patterns of protein kinase dysregulation in CD4+ T cells in pathogenic and apathogenic SIV infection. Abstract #78, 19th Annual Symposium on Nonhuman Primate Models of AIDS, San Juan PR, Nov 7-10, 2001.


    F Villinger, GT Brice, AE Mayne, P Bostik, K Mori, CH June and AA Ansari. Adoptive transfer of SIV na¿ve autologous CD4+ cells is sufficient to induce a longterm non progressor status. Abstract #14, 19th Annual Symposium on Nonhuman Primate Models of AIDS, San Juan PR, Nov 7-10, 2001.


    SL Buge, M Ma, R Amara, L Wyatt, P Earl, B Grimm, F Villinger, J Altman, B Moss, SI Staprans, NL Kozyr, DC Montefiori, Y Xu, S O'Neil, JG Herndon, HM McClure, H Robinson and J McNicholl. Includsion of a protein booster in a DNA priming and MVA boosting protocol for an AIDS vaccine. Abstract #228, AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001.


    R Amara, J Smith, F Villinger, J Altman, S Staprans, N Kozyr, S Lydy and H Robinson. Better control of a pathogenic SHIV89.6p challenge by Gag-Pol-Env than Gag-Pol DNA/MVA vaccine: Requirement forincludion of envelope in AIDS vaccines. Abstract #165 AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001.


    F Villinger, V Bostik, AE Mayne, P Bostik, D Adams, CN Kim, RA Otten, W Heneine, WM Switzer, F Novembre, HM McClure, TM Folks and AA Ansari. SIVhu, a naturally attenuated SIVsm isolate is apathogenic and immunogenic in newborn macaques. Keystone Symposium on Experimental AIDS Vaccines, Keystone CO, Apr 4-10, 2001.


    F Villinger, V Bostik, AE Mayne, P Bostik, D Adams, CN Kim, RA Otten, W Heneine, WM Switzer, F Novembre, J DeRosayro, HM McClure, TM Folks and AA Ansari. Apathogenicity and protective immune responses in newborns inoculated with the naturally attenuated SIVhu isolate. Dan Pallota Meeting on Experimental AIDS Vaccines, Los Angeles CA, June 27-28, 2001.


    F Villinger, GT Brice, AE Mayne, P Bostik, K Mori, CH June and AA Ansari. Transfusion of pre-SIV infection collected autologous CD4+ T cells is sufficient to induce a longterm non-rogressor status in chronically infected rhesus monkeys. Abstract #D3e. AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001.


    AA Ansari, AE Mayne, P Bostik, JB Sundstrom and F Villinger. IL-12 induces potent antiviral responses during acute SIV infection. Abstract #. AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001.


    Y Tang, RR Amara, F Villinger, S Staprans, N Kozyr, B Grimm, J Smith, S Lydy and HL Robinson. High leves of nonfunctional proviral DNA in DNA/MVA vaccinated animals with controlled viral infections. Abstract #156 AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001.


    E O'Neill, I Martinez, Z Israel, F Villinger, M Sidhu, R Stout, D Montefiori, AA Ansari, M Martinez and E Kraiselburd. Protection by SIV VLP DNA prime/protein boost following mucossal SIV challenge is markedly enhanced by IL-12/GM-CSF coadministration. Abstract #159 AIDS Vaccine 2001 Philadelphia PA, Sept 5-8, 2001


    TR Gibb, S Bavari, F Villinger, N Woolen and EA Henchal. Characterization of early diagnostic markers of filoviral infection in the non-human primate model. U.S. Army Military Veterinary Medical Seminar, San Antonio, March 25-30, 2000.


    P Bostik, F Villinger, KP Greenberg, A Mayne and AA Ansari. Mechanisms of CD4+ T cell anergy resistance in SIV disease resistant sooty mangabeys - Possible role of an IL-2 promoter mutation. Abstract #78, 18th Annual Symposium on Nonhuman Primate Models of AIDS, Madison WI, Oct 4-7, 2000.


    F Villinger, K Mori, Y Yasutomi, K Sugama, S Sawada, K Ueberla, H Ruebsamen-Waigmand, AE Mayne, P Bostik and AA Ansari. Post but nor pre-SHIV-RT infection chemotherapy of monkeys with a NNRT inhibitor induces protective responses against heterologous SHIV89.6p challenge. Abstract #19, 18th Annual Symposium on Nonhuman Primate Models of AIDS, Madison WI, Oct 4-7, 2000.


    CJ Weber, SA Safley, F Villinger, C Cohen, EH Jackson, LB Dirksen, and JA Kapp. Interleukin-6 (IL-6) Production by Human Parathyroids. Annual Meeting of the American Association of Endocrine Surgeons, June 2000.


    P Bostik, G Brice, AE Mayne F Villinger, MG Lewis and AA Ansari. Telomerase activity and proliferation of CD4+ T cells in SIV infected nonhuman primate species: Inverse association with disease progression. Abstract 304, Keystone Symposia on Molecular and Cellular Biology: AIDS Pathogenesis, Keystone, CO January 7-13, 1999.


    S Safley, C Weber, J Kapp, F Villinger, A Brooks, L Dirksen, J Orkin and AA Ansari. Rapid destruction of intraperitoneal (IP) microencapsulated (MC) pig islets by streptozotocin (SZN) diabetic monkeys. Abstract, Seventh World Congress of the International Pancreas and Islet Transplant Association, Sydney (Australia) August 22-25, 1999.


    S Safley, C Weber, J Kapp, F Villinger, A Brooks, L Dirksen, J Orkin and AA Ansari. Rapid destruction of intraperitoneal (IP) microencapsulated (MC) pig islets by streptozotocin (SZN) diabetic monkeys. Abstract, Seventh World Congress of the International Pancreas and Islet Transplant Association, Sydney (Australia) August 22-25, 1999.


    P Lena, P Luciw, M Gardner, J Smith, M McChesney, F Villinger, G Rhodes. Altered Patterns of SIVmac251 Infection after DNA Vaccination in Macaques. Abstract, Second Annual Conference on Vaccine Research, Bethesda, March 1999.


    P Lena, F Villinger, L Giavedoni, G Rhodes and P Luciw. Rhesus cytokine expression vectors as adjuvants for immunization. Abstract 54, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    F Villinger, A Mayne, P Bostik, NF Chikkala, AA Ansari, HM McClure, WM Switzer, R Otten, W Heneine and TM Folks. Induction of longterm protective effect against heterologous challenge in SIVhu infected macaques. Abstract 39, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    F Villinger, K Mori, S Sawada, K Sugama, H Ruebsamen-Waigmann and AA Ansari. High viral replication of SHIV-RT in vivo leads to the emergence of mutants partially resistant to non-nucleosidic reverse transcriptase treatment. Abstract 143, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    K Mori, Y Yasutomi, S Sawada, F Villinger, K Sugama, B Rosenwirth, K Ueberla, S Yamazaki, A Ansari, H Ruebsamen-Waigmann. Post-exposure Prophylaxis of SHIV-RT infected monkeys with non-nucleoside RT inhibitor GW420867 suggested importance of induction and maintenance of viral specific cell mediated immune responses. Abstract 111, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    77. GH Rhodes, MB McChesney, PA Luciw, JV Torres, MB Gardner, F Villinger, AA Ansari, K Lee, RW Malone, P Lena, J Smith, J Welsh and PM Montbriand. Nucleic acid vaccination with a single SIV can protect Rhesus macaques from oral challenge with pathogenic SIVMAC239. Abstract 67, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    79. P Bostik, G Brice, AE Mayne F Villinger, MG Lewis and AA Ansari. Telomerase activity and proliferation of CD4+ T cells in SIV infected nonhuman primate species: Inverse association with disease progression. Abstract 304, Keystone Symposia on Molecular and Cellular Biology: AIDS Pathogenesis, Keystone, CO January 7-13, 1999.


    AA Ansari, A Mayne, P Bostik and F Villinger. Does resistance in naturally SIV infected sooty mangabeys rely on reduced requirements for second signals in CD4+ T cell activation. Abstract 33, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    81. NL Haigwood, JD Bradshaw, K Koo, SP Mossman, L Kuller, D Anderson, R Bucala, RG Andrews, A Troutt, F Villinger, SL Hu and YD Zhu. Macaque blood-derived dendritic cells and fibrocytes as antigen-presenting cells for HIV-vaccines. Abstract 38, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    S Buch, F Villinger and O Narayan. Requirement of IL-4 for productive SHIV replication in macaque macrophages and brain. Abstract 88, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    P Bostik, JB Sundstrom, KP Greenberg, F Villinger and AA Ansari. In vitro tropism of various HIV/SIV strains for different primary endothelial cells and its potential significance in vivo. Abstract 95, Seventeenth Annual Symposium on Nonhuman Primate Models for AIDS, New Orleans LA, Oct 6-9, 1999.


    WM Switzer, V Shanmugam, A Wright, F Villinger and W Heneine. Phylogenetic analysis of new simian foamy viruses from African and Asian nonhuman primates. International Symposia on Foamy Viruses, Paris, June 1999.


    GL Giambartolomei, F Villinger, B Lasater and VA Dennis. Lack of B7.1 gene expression correlates with antigen-specific unresponsiveness in rhesus monkeys with lymphatic filariasis. Symposium of the American Association for Tropical Medicine, 1998.


    78. PH Van der Meide, F Villinger, AA Ansari, MK Gately, H Schellekens and J Wijdenes. Interleukin-12 strongly potentiates the candidate vaccine molecule HIV-1 gp120 in Rhesus monkeys. Abstr 231, Second Joint Meeting of the ICS and ISICR, Jerusalem, Israel Oct 25-30, 1998 ad Eur Cytokine Netw, Vol 9 p 537, 1998.


    PH Van der Meide, F Villinger, AA Ansari, MK Gately, H Schellekens and J Wijdenes. Interleukin-12 strongly potentiates the candidate vaccine molecule HIV-1 gp120 in Rhesus monkeys. Abstr 231, Second Joint Meeting of the ICS and ISICR, Jerusalem, Israel Oct 25-30, 1998 ad Eur Cytokine Netw, Vol 9 p 537, 1998.


    P Bostik, G Brice, AE Mayne F Villinger, MG Lewis and AA Ansari. Increased proliferation of CD4+ T lymphocytes in different species of SIV infected nonhuman primates is associated with lack of, or delayed disease progression. Abstract 66, Sixteenth Symposium on Nonhuman Primate Models for AIDS, Atlanta GA, Oct 7-10 1998.


    PH Van der Meide, F Villinger, MK Gately, RJ Groenestein, MCDC de Labie, WH Koornstra, J Heeney and J Wijdenes. Interleukin-12 strongly potentiates the immunogenicity of the candidate vaccine molecule HIV-1gp120 in rhesus monkeys. Meeting of the British Cytokine Society, June 18, 1998.


    SZ Bucur, TW Gillespie, ME Lee, JA Adams, RA Bray, F Villinger, EF Winton, FJ Novembre, AA Ansari and CD Hillyer. Hematopoietic response to rhGET administration during early versus late stage SIV infected rhesus macaques. Abstract 24, Sixteenth Symposium on Nonhuman Primate Models for AIDS, Atlanta GA, Oct 7-10 1998.


    F Villinger, NF Chikkala, SS Brar, SZ Bucur, ME Lee, TW Gillespie, JA Adams, P Bostik, AE Mayne, P Dailey, FJ Novembre, AA Ansari and CD Hillyer. IL-12 responses in vivo are lost during disease progression in SIV infected macaques. Abstract 14, Sixteenth Symposium on Nonhuman Primate Models for AIDS, Atlanta GA, Oct 7-10 1998.


    PH Van der Meide, F Villinger, MK Gately, RJ Groenestein, MCDC de Labie, WH Koornstra, J Heeney and J Wijdenes. Interleukin-12 strongly potentiates the immunogenicity of the candidate vaccine molecule HIV-1gp120 in rhesus monkeys. Meeting of the British Cytokine Society, June 18, 1998.


    SZ Bucur, TW Gillespie, ME Lee, JA Adams, RA Bray, F Villinger, EF Winton, FJ Novembre, AA Ansari and CD Hillyer. Bone marrow response to rrIL-3 administration in SIV infected rhesus macaques is related to stage of infection. Abstract 96, Sixteenth Symposium on Nonhuman Primate Models for AIDS, Atlanta GA, Oct 7-10 1998.


    F Villinger, SS Brar, AE Mayne, NF Chikkala, J Winter, GT Brice, CD Hillyer and AA Ansari. IFN-g AND IL-2 antigen specific PBMC responses are lost shortly after SIV infection. Abstract #101, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    GT Brice, A Mayne, CD Hillyer, F Villinger and AA Ansari. Ligation of costimulatory molecules CD28 or CD40L inhibits SIVmac251 replication in CD4+ T cells from rhesus macaques and sooty mangabeys. Abstract #123, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    SZ Bucur, ME Lee, SE Resnick, DA Lackey III, JW Adams, F Villinger, A Mayne, LS Buccolo RA Bray, EF Winton, F Novembre, AA Ansari and CD Hillyer. Differential bone marrow response to rhFlt-3 ligand administration in early versus late stage SIV infected rhesus macaques. Abstract #78, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    P Bostik, F Villinger, SS Brar, GT Brice, JA Adams, CD Hillyer and AA Ansari. Equivalent and/or enhanced inhibition of SIVmac251 infection/replication by amino-terminal modified chemokines. Abstract #29, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    Z Smit-McBride, J Mattapallill, F Villinger, M McChesney, J Collins, P Dailey and S Dandekar. Prevalence of TH1 response in intestinal mucosa in comparison to peripheral mononuclear cells in SIV infection. Abstract #33, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    F Villinger, NF Chikkala, SS Brar, SZ Bucur, ME Lee, DA Lackey III, JA Adams, P Bostik, AE Mayne, AA Ansari and CD Hillyer. In vivo IL-12 response is maintained in asymptomatic macaques infected with SIVmac239. Abstract #100, Fifteenth Annual Symposium on Nonhuman Primate Models of AIDS, Seattle, WA, Oct 1997.


    F Villinger, SS Brar, AE Mayne, NF Chikkala, G Brice and AA Ansari. Early loss of antigen induced IFNg with gradual increase in IL-6 in macaques infected with SIVmac251 as they progress towards AIDS. Ninth Annual Meeting of the National Cooperative Vaccine Development Groups (NCDVGs) for AIDS, Bethesda MD, May 4-7, 1997.


    CD Hillyer, F Villinger, GT Brice, JW Adams, DA Lackey, ME Lee, A Mayne, SS Brar and A Ansari. Influence of recombinant rhesus (rr) IL-16 on the activation of CD4+ T-cells and SIV replication in vitro. Abstract 77, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    Z Smit-McBride, F Villinger, E Jones, C Oxford, C Miller, A Ansari and S Dandekar. Alterations in gd T cell-subsets in intestinal epithelial tissue and peripheral blood lymphocytes in SIV infection. Abstract 68, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    GT Brice, ER McLaughlin, F Villinger, CD Hillyer, A Mayne and A Ansari. Differential dose response effects on SIV replication enhancement or inhibition by chemokines RANTES, MIP-1a and MIP-1b. Abstract, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    CD Hillyer, DA Lackey, SZ Bucur, JW Adams, ME Lee, LS Buccolo, LD Trainor, TW Gillespie, F Villinger, A Mayne, RA Bray, EF Winton and A Ansari. Studies of the effects of recombinant human (rh) and rhesus (rr) cytokines on hematologic and virologic parameters in a cohort SIVmac239 infected macaques and in vitro. Abstract 78, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    MA Kassem, AA Ansari, FA El-Samannoudy, A Mayne, F Villinger, D Hunt, JB Sundstrom and SS Brar. Immunohistological and molecular analysis of cell adhesion molecules in cardiac biopsies of patients post-transplantation. Teh third Conference of the Egyptian Association of Immunologists, Cairo March 28-29, 1996.


    F Villinger, SS Brar, GT Brice, NF Chikkala, F Novembre, A Mayne, CD Hillyer and A Ansari. Decrease in the secretion of chemokines in PHA/IL-2 stimulated PBMC from HIV-1 infected chimpanzees as they progress towards AIDS. Abstract 19, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    F Villinger, NF Chikkala, J Winter, A Mayne, M Hemphill, T Ziegler, CD Hillyer and A Ansari. Primary influenza pCTL response: A potential parameter to predict SIV disease course in rhesus macaques? Abstract 70, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    A Ansari, J Winter, A Mayne, CD Hillyer. A Brodie-Hill, TM Folks, SS Brar, PJ Dailey and F Villinger. Naturally CD4 negative macaque and mangabeys present normal responses to antigen and hematopoiesis but differ in their response to SIV infection. Abstract 53, 14th Annual Symposium on Nonhuman Primate Models of AIDS, Portland, OR, Oct 23-26, 1996.


    A Ansari, A Mayne, G Brice, J Sundstrom and F Villinger. Identification of unique intracellular phosphorylated proteins in activated T cells from rhesus macaques and sooty mangabeys. Abstract 15, 13th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey, CA, Nov 5-8, 1995.


    PA Luciw, T Low, C Mandell, M Stout, A Ansari and F Villinger. Pathogenesis by replication-competent simian immunodeficeincy virus (SIV) vector expressing the interleukin-2 gene. Abstract 27, 13th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey, CA, Nov 5-8, 1995.


    C Heise, F Villinger, A Ansari, S Brar, T Faith and S Dandekar. IL-10 expression is upregulated in vivo during acute SIV infection. Abstract 6, 13th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey, CA, Nov 5-8, 1995.


    F Villinger, SS Brar, NC Chikkala, A Mayne and A Ansari. Human and nonhuman primate cytokines are almost identical yet they present biological and antigenic differences. Abstract 93, 13th Annual Symposium on Nonhuman Primate Models of AIDS, Monterey, CA, Nov 5-8, 1995.


    C Heise, F Villinger, J Stone, S Brar, AA Ansari and S Dandekar. Cytokine mRNA and cell adhesion molecule expression in intestinal mucosa of rhesus macaques infected with pathogenic or nonpathogenic SIV. West Coast Retrovirus Meeting, Newport Beach CA, Oct 6-8, 1994.


    S Dandekar, C Heise, J Stone, F Villinger, AA Ansari and M Elices. Cell adhesion molecule expression and cytokine mRNA in intestinal mucosa of SIV infected rhesus macaques. Twelvth Annual Symposium on Nonhuman Primate Models for AIDS. Boston, MA, Oct 12-15, 1994.


    T Neal, HK Holland, F Villinger, AA Ansari, Baum CM and WH Fleming. CD34+ progenitors are not infected by HIV-1 in the majority of asymptomatic seropositive patients. Thirty sixth Annual Meeting of the American Society of Hematology, Nashville, TN, Dec 2-6, 1994.


    F Villinger, BA Faraj, ZL Olkowski, RT Jackson and AA Ansari. Functional expression of a serotonin transporter in human peripheral lymphocytes. Seventy eight Annual Meeting of the Federation of American Societies for Experimental Biology, Annaheim, CA, April 24-28, 1994.


    JB Sundstrom, F Villinger and AA Ansari. Modulation of the expression of CAMs on the surface of HIV- and SIV-infected PBMC. Seventy eight Annual Meeting of the Federation of American Societies for Experimental Biology, Annaheim, CA, April 24-28, 1994


    AA Ansari, F Villinger, A Mayne and TM Folks. Cytokine analysis and studies of apoptosis of unfractionated and cloned T cell lines from SIV-infected nonhuman primates. Workshop on HIV/SIV Pathogenesis and Mucosal Transmission, Bethesda MD, March 14-17, 1994.


    M Singh-Jairam, J McCormack, F Villinger, and G Rodey. Use of quantitative PCR to detect prior alloimmunization in solid organ transplant recipients. Twentieth Annual Meeting of the American Society for Histocompatibility and Immunogenetics, Pittsburgh, PA, Oct 21-26, 1994.


    F Villinger, T Jehuda-Cohen, JD Powell, A Mayne, F Finkelman, F Novembre and AA Ansari. In vivo polyclonal activation reveals occult SIV infection. Twelvth Annual Symposium on Nonhuman Primate Models for AIDS. Boston, MA, Oct 12-15, 1994.


    AA Ansari, Mayne A and F Villinger. Increased susceptibility for apoptosis in SIV infection: Potential mechanisms and pathways. Twelvth Annual Symposium on Nonhuman Primate Models for AIDS. Boston, MA, Oct 12-15, 1994.


    V Courgnaud, I Fossati, F Villinger and P Sonigo. Adaptation and pathogenicity of SIV from sooty mangabey monkey. Fifth Workshop on the Pathogenesis of Animal Retroviruses, La Rochelle (F), October 28-31, 1993.


    AA Ansari, A Mayne, D Hunt and F Villinger. TH1/TH2 subset analysis in PBMC samples of sooty mangabeys and rhesus macaques naturally and experimentally infected with SIV. Eleventh Annual Symposium on Nonhuman Primate Models for AIDS, Madison WI, Sept. 19-22, 1993.


    F Villinger, S Lauro, A Mayne TM Folks and AA Ansari. Sequence analysis of the SIVsmm Nef gene in naturally and experimentally infected sooty mangabey monkeys and macaques. Eleventh Annual Symposium on Nonhuman Primate Models for AIDS, Madison WI, Sept. 19-22, 1993.


    A Mayne, F Villinger, H Findley and AA Ansari. Quantitative differences in the levels of IL-2 and IFNg produced by PBMC of naturally SIV infected sooty mangabeys and rhesus macaques experimentallly infected with SIV. Eleventh Annual Symposium on Nonhuman Primate Models for AIDS, Madison WI, Sept. 19-22, 1993.


    S Buchbinder, D Mann, L Louie, F Villinger, M Katz, S Holmberg et al. Healthy long-term positives (HLPs): Genetic cofactors for delayed HIV disease progression. IXth International Conference on AIDS, Berlin, June 7-11, 1993.


    F Villinger, S Lauro, D Clark, TM Folks and AA Ansari. Does the Nef gene contribute to the disease resistance of sooty mangabeys (smm) naturally infected with SIVsmm? Keystone Symposia: Molecular Immunology of Virus Infection, March 17-24, 1993.


    F Villinger. TH0, TH1 and TH2 paradigm in SIV induced infection and disease. The First Conference on Human Retroviruses and Related Infections, Washington DC, December 12-16, 1993.


    F Villinger, S Lauro, TM Folks and AA Ansari. Does sequence diversity of the Nef gene contribute to the disease resistance and susceptibility to SIV infection in naturally infected mangabeys and experimentally infected macaques? Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    D Hunt, M Vuchetich, F Villinger, H Findley, A Mayne, TM Folks and AA Ansari. Establishment of quantitative cytokine assays and detection of mRNA coding for the cytokines in lymphoid cells from rhesus macaques and sooty mangabeys. Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    A Mayne T Jehuda-Cohen, F Villinger and AA Ansari. Specificity, sensitivity, and reproducibility for the detection of SIV-specific antibodies in the serum and supernatant fluid of PWM-stimulated PBMC from mangabeys and macaques. Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    AA Ansari, A Mayne, D Hunt and F Villinger. Analysis of the frequencies of TH0, TH1 and TH2 subsets of cloned lymphoid cells from mangabeys and macaques. Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    M Isahakia, RA Aman, F Villinger, A Mayne, T Jehuda-Cohen, JD Powell, M Vuchetich, MA Suleman, A Chege, I Chirchir RB Lal and AA Ansari. SIV and STLV-I seroprevalence in East African nonhuman primate species. Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    F Villinger, TM Folks, A Mayne, M Vuchetich, H Findley and AA Ansari. Analysis of the TNFb gene in rhesus macaques and sooty mangabeys. Tenth Symposium on Nonhuman Primate Models for AIDS, Porto Rico, November 10-14, 1992.


    F Villinger, EV Genovesi, M Juarero-Gonzales, TC Whyard and RC Knudsen. Surface reaction of African swine fever virus (ASF)-monoclonal antibodies (mabs) and immune swine serum antibodies (IS) to ASF-infected pig monocytes (MO). Abstract S-27. Abstracts of the 91st General Meeting of the American Society for Microbiology, Dallas, May 5-9, 1991.


    F Villinger, T Jehuda-Cohen, JD Powell, TM Folks, M Vuchetich, E Lockwood, KW Sell and AA Ansari. Natural transmission of SIVsmm between nonhuman primates transmits predominantly different isolates than experimental transmission with in vitro propagation of the virus. Seventy fifth Annual Meeting of the Federation of American Societies for Experimental Biology, Atlanta, GA, April 21-25, 1991.


    T Jehuda-Cohen, F Villinger, HM McClure, JD Powell, KW Sell and AA Ansari. Can SIV seronegative yet infected blood transmit infection? Seventy fifth Annual Meeting of the Federation of American Societies for Experimental Biology, Atlanta, GA, April 21-25, 1991.


    JD Powell, DP Bednarik, T Jehuda-Cohen, F Villinger, TM Folks and AA Ansari. Regulation of immune activation/retroviral replication by CD8+ T cells. Seventy fifth Annual Meeting of the Federation of American Societies for Experimental Biology, Atlanta, GA, April 21-25, 1991.


    CD Hillyer, DA Lackey, F Villinger, EF Winton, AA Ansari and HM McClure. CD34+ progenitor cell, colony forming unit-granulocyte/ macrophage (CFU-GM) and burst forming unit-erythroid (BFU-E) analysis in SIVsmm infected Rhesus macaques (RM); Functional bone marrow abnormalities without evidence of direct stem cell infection. Ninth Symposium on Nonhuman Primate Models for AIDS, Seattle, WA, November 6-8, 1991.


    F Villinger, B De, T Jehuda-Cohen, JD Powell, N Neckelmann, HM McClure, TM Folks, and AA Ansari. Both in vitro culture and in vivo passage lead to selective replication of SIV variants. Eigth Symposium on Nonhuman Primate Models for AIDS, Abstract 2, New Orleans, LA, October 28-30, 1990.


    HM McClure, DC Anderson, PN Fultz, AA Ansari, T Jehuda-Cohen, F Villinger, SA Klumpp, W Switzer, E Lockwood and A Brodie. Maternal transmission of SIVsmm in rhesus macaques. Eigth Symposium on Nonhuman Primate Models for AIDS, Abstract 18, New Orleans, LA, October 28-30, 1990.


    M Vuchetich, T Jehuda-Cohen, F Villinger, JD Powell, HM McClure and AA Ansari. Evidence that the seronegative sooty mangabeys in our colony are infected with SIVsmm. Eigth Symposium on Nonhuman Primate Models for AIDS, Abstract 82, New Orleans, LA, October 28-30, 1990.


    T Jehuda-Cohen, F Villinger, HM McClure, E Lockwood, JD Powell, and AA Ansari. Transmission of SIV infection by transfusion of seronegative blood in nonhuman primates. Eigth Symposium on Nonhuman Primate Models for AIDS, Abstract 21, New Orleans, LA, October 28-30, 1990.


    KW Sell, T Jehuda-Cohen, B Slade, J Powell, F Villinger, H McClure, A Ansari. Detection of HIV-1 infected inviduals in a seronegative high-risk population by polyclonal B cell activation in vitro. Sixth International Conference on AIDS, San Francisco, CA, June 20-24, 1990.


    JD Powell, DP Bednarik, TM Folks, T Jehuda-Cohen, F Villinger, HM McClure and AA Ansari. CD8+ T-cell-induced inhibition of retroviral replication is mediated at the transcriptional level. Eigth Symposium on Nonhuman Primate Models for AIDS, Abstract 27, New Orleans, LA, October 28-30, 1990.


    EV Genovesi, F Villinger, DJ Gerstner and EV Kramer. African swine fever virus inhibition in cultured swine blood monocytes (MO) by phosphonoacetic acid (PAA) and phosphonoformic acid (PFA). Abstract P72. Seventy-first Conference of Research Workers in Animal Disease, Chicago, November 1990.


    F Villinger, EV Genovesi, DJ Gerstner, TC Whyard and RC Knudsen. Swine monocytes/macrophages can be stimulated by the murine macrophage growth factor CSF-1 in vitro: Use in a virus-cell model for investigating African swine virus. Eighty-ninth Annual Meeting of the American Society for Microbiology, New Orleans, May 14-18, 1989.


    AA Ansari, JD Powell, T Jehuda-Cohen, F Villinger, D Anderson and HM McClure. Delineation of peptide specific T cell immunity in SIV/SMM infected nonhuman primates using the Cellular Western blot assay. Seventh Symposium on Nonhuman Primate Models for AIDS, Portland, OR, October 11-13, 1989.


    JD Powell, T Jehuda-Cohen, F Villinger, DC Anderson, HM McClure and AA Ansari. CD8+ T cells from SIV seronegative sooty mangabeys suppress SIV/SMM virus replication in vitro. Seventh Symposium on Nonhuman Primate Models for AIDS, Portland, OR, October 11-13, 1989.


    F Villinger, T Jehuda-Cohen, JD Powell, DC Anderson, HM McClure and AA Ansari. Relative differences in the regulation and susceptibility for the replication of SIV/SMM in CD4+ T cells and monocytes from asymptomatic mangabeys and disease susceptible rhesus macaques. Seventh Symposium on Nonhuman Primate Models for AIDS, Portland, OR, October 11-13, 1989.


    T Jehuda-Cohen, B Slade, JD Powell, F Villinger, DC Anderson, HM McClure, KW Sell and AA Ansari. A novel method for the detection of antibodies against SIV and HIV in conventionally seronegative sooty mangabeys and high risk humans. Seventh Symposium on Nonhuman Primate Models for AIDS, Portland, OR, October 11-13, 1989.


    M Ackermann, M Palatini, N Berger, F Stampfli, HK Mueller, M Hoffmann, M Knuchel, R Zanoni, F Villinger, L Bruckner and U Kihm. Seroepidemiological survey of virus diseases in the swiss pig population from 1985 to 1988: Screening for the prevalence of pigs carrying antibodies against Pseudorabies virus (PRV), Transmissible Gastroenteritis (TGE), Swine Influenza virus, Swine Vesicular Disease (SVD), African Swine Fever (ASF), Classical Swine Fever (CSF), and Porcine Epidemic Diarrhoea (PED). First World Congress of W.A.V.M.I. Microbiology, Immunology and Animal Infectious Diseases, Lyon (France), July 25-29 1988.


    F Villinger, EV Genovesi, DJ Gerstner, TC Whyard and RC Knudsen. African swine fever virus susceptibility of swine blood monocytes maintained in vitro by the murine macrophage-specific growth factor CSF-1. Sixty-ninth Conference of Research Workers in Animal Disease, Chicago, November 14-15, 1988.


    F Villinger, EV Genovesi, TC Whyard, DJ Gerstner and RC Knudsen. An in vitro mononuclear cell culture model to study African swine fever virus (field isolates) infection: Susceptibility of CSF-1 propagated swine mononuclear phagocytes to ASFV infection. African swine fever and pig immunology meeting, Salamanca (Spain), November 1-5 1987.


    F Villinger, HK Mueller, M Ackermann, L Bruckner and U Kihm. An ELISA for the detection of antibodies to a bioengineered VIA-antigen. 17th Conference of the O.I.E. Foot and Mouth Disease Commission. Working Document pp 175-187, Paris, Oct. 1986.


    Thesis for obtaining a Doctor of Veterinary Medicine: Serologische Untersuchungen zur Maul- und Klauenseuche: Nachweis von Antikoerpern gegen biotechnologisch hergestelltes "Virus Infection Associated" (VIA) Antigen mittels ELISA und Nachweis neutralisierender Antikoerper in der jaehrlich geimpften Schweizerischen Rinderpopuplation. (Serologic surveys on Foot-and-Mouth disease: Detection of antibodies to biotechnologically produced "Virus Infection Associated" (VIA) antigen with ELISA and detection of neutralizing antibodies in the Swiss yearly vaccinated cattle population.) Aus dem Eidgenoessischen Vakzine-Institut, Basel und dem Institut fuer Virologie der Universitaet Zuerich, (Switzerland), 1986.F Villinger, HK Mueller, M Ackermann, L Bruckner, and U Kihm. Detection of antibodies to foot and mouth disease (FMD) virus infection associated (VIA) antigen with an ELISA. IV International Symposium of Veterinary Laboratory Diagnosticians, Amsterdam, June 1986, pp. 810-812.