Pathology Faculty Member

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    Experimental Pathology
    105-B Whitehead Bldg., 615 Michael St.
    Atlanta, GA 30322
    404 727-8536 Phone

    For an expert Pathology consultation,
    or to send a specimen, call:

    1-404-712-5947


    Charles A Parkos, MD, PhD

    Epithelial Pathobiology
    Immunology
    Gastrointestinal Pathology

    Professor
    Pathology & Laboratory Medicine


    Pathology Faculty Photo

    Other Appointments/Positions

    Director, Medical Scientist Training Program, Emory

    Professor & Vice Chair, Emory

    Co- Director, Emory Medical Scientist Training Program, Emory University

    Member, Graduate Training Program in Immunology and Molecular Pathogenesis, Emory University

    Member, Winship Cancer Center, Emory University School of Medicine, Emory University

    Professor of Pathology, tenured, Emory University


    Pathology Division(s):
    Anatomic Pathology
    Experimental Pathology


    Email Address:
    cparkos@emory.edu


    Education:

    B.A   University of California at San Diego, La Jolla, CA, - 1979

    M.D.   University of California at San Diego, La Jolla, CA, - 1987

    Ph.D   Scripps Clinic and Research Foundation, University of California at San Diego, La Jolla, CA, - 1987



    Research Interests:

    - Pathobiology of leukocyte interactions with mucosal epithelia
    Studies in my lab are focused on two areas including the molecular basis of interactions between leukocytes and mucosal epithelia and how such interactions regulate the function of epithelial cells. Specifically, we are exploring how epithelial cells respond to migrating innate immune cells (neutrophils) and on defining the proteins that regulate transmigration of leukocytes across mucosal surfaces that line organs in the gastrointestinal tract and lung. We are particularly interested in the nature of epithelial counterreceptors for migrating neutrophils. Indeed, our recent observations point to a growing importance for a family of transmembrane intercellular junction adhesion molecules termed JAMs that have been shown to function in the maintenance of epithelial barrier and serve as receptors for both migrating leukocytes and viral pathogens. The functional biology of these recently described receptors is just becoming appreciated where we have combined our expertise in epithelial cells and structural/molecular biology to elucidate structure-function relationships in JAMs. We are also studying factors that regulate how fast leukocytes migrate through tissues by modeling how microbial products that activate Toll-like receptors and chemotactic receptors regulate leukocyte transepithelial migration. Our results have linked control of the rate of leukocyte migration to signaling events that occur after ligation of a cell surface protein termed CD47 by its receptor signal regulatory protein alpha (SIRPalpha). These studies directed at understanding the molecular basis of mucosal inflammation have begun to provide new ideas for anti-inflammatory strategies that are tissue-specific and the opportunity for many collaborative interactions within the Emory community.



    Clinical Focus:

    I am a practicing Surgical Pathologist and Director of the Division of Gastrointestinal Pathology at Emory University Hospital and my clinical focus is limited exclusively to Diagnostic Surgical Pathology of the Gastrointestinal-Tract and Hepatobiliary system.


    Honors / Awards:

    Ad Hoc NIDDK special emphasis panel

    , 2005-Present

    Program Chair elect, American Society of Investigative Pathology, 2005-2008

    Member, American Association of University Pathologists (Pluto Society)

    , 2004-Present

    Ad hoc NIDDK study section member for training and career development applications, 2003

    Dean's Senior Clinical Investigators Award, 2000-Present

    Study Section Member, Crohn's and Colitis Foundation, 2000-Present

    Arthritis Foundation Biosciences Award, 1997-2000

    Study Section Member, Biochemistry, Arthritis Foundation, 1997-2000

    Arie and Ida Crown Memorial Foundation Award; Crohn's and Colitis Foundation of America, 1994-1995

    Crohn's and Colitis Foundation Young Investigator Award, 1991

    Chief Resident in Pathology, Brigham and Womens Hospital, Boston, 1990

    A Baird Hastings Honor Society Charter Member, UCSD School of Medicine, 1981-1987

    Specialty Boards:

    1993  American Board of Pathology, Anatomic Pathology


    Selected Publications:

    Published and Accepted Research Articles -

    Samarin SN, Ivanov AI, Flatau G, Parkos CA, Nusrat A. 2007. Rho/ROCK-II signaling mediates disassembly of epithelial apical junctions. Mol. Biol. Cell. 18:3429-39


    Zimring JC, Cadwell CM, Chadwick TE, Spitalnik SL, Schirmer DA, Wu T, Parkos CA, Hillyer CD. 2007. Non-hemolytic antigen loss from RBCs requires cooperative binding of multiple antibodies recognizing different epitopes. Blood. In-press


    Wilkinson RA, Evans JR, Jacobs JM, Slunaker D, Pincus SH, Pinter A, Parkos CA, Burritt JB, Teintze M. 2007. Peptides selected from a phage display library with an HIV-neutralizing antibody elicit antibodies to HIV gp120 in rabbits, but not to the same epitope.AIDS Reseach. In-press


    Lebeis, SL, Bommarius B, Parkos CA, Sherman MA, Kalman D. 2007. TLR signaling mediated by myd88 is required for a protective innate immune response by PMN to citrobacter rodentium. J. Immunol. 179:566-77


    Ivanov AI, Bachar M, Babbin BA, Adelstein RS, Nusrat A, Parkos CA. 2007. A unique role of nonmuscle myosin heavy chain IIA in regulation of epithelial apical junctions. PLoS One 2:e658


    Wang Z, Wade P, Mandell KJ, Akyildiz A, Parkos CA, Mrsny RJ, Nusrat A. 2007. Raf 1 represses expression of the tight junction protein occludin via activation of the zinc finger transcription factor Slug. Oncogene;26:1222-30


    Wang Z, Wade P, Mandell KJ, Akyildiz A, Parkos CA, Mrsny RJ, Nusrat A. 2007. Raf 1 represses expression of the tight junction protein occludin via activation of the zinc finger transcription factor Slug. Oncogene;26:1222-30


    Sircar M, Bradfield PF, Aurrand-Lions M, Fish RJ, Alcaide P, Yang L, Newton G, Lamont D, Sehrawat S, Mayadas T, Liang TW, Parkos CA, Imhof BA, Luscinskas FW. 2007 Neutrophil Transmigration under Shear Flow Conditions In Vitro Is Junctional Adhesion Molecule-C Independent. J Immunol. 178(9):5879-5887.


    Mandell KJ, Berglin L, Severson E, Edelhauser HF, Parkos CA. 2007. "Expression of JAM-A in the Human Corneal Endothelium and Retinal Pigment Epithelium: Localization and Evidence for Role in Barrier Function". Invest Ophthalmol Vis Sci, In Press


    Babbin BA, Parkos CA, Mandell KJ, Winfree LM, Laur O, Ivanov AI, Nusrat A. 2007 . Annexin 2 Regulates Intestinal Epithelial Cell Spreading and Wound Closure through Rho-Related Signaling. Am J Pathol. 170:951-66.


    Ivanov AI, McCall IC, Babbin B, Samarin SN, Nusrat A, Parkos CA. 2006. Microtubules regulate disassembly of epithelial apical junctions. BMC Cell Biol. Mar 1;7:12


    Babbin BA , Lee WY , Parkos CA , Winfree LM , Akyildiz A , Perretti M , Nusrat A . 2006. Annexin I regulates SKCO-15 cell invasion by signaling through formyl peptide receptors. 281(28):19588-99.


    Mandell KJ, Holley GP, Parkos CA and Edelhauser HF. 2006. Junctional adhesion molecule-A is expressed in tight junctions of the rabbit corneal endothelium and regulates corneal swelling. Invest Ophthalmol Vis Sci. 47(6):2408-16


    Bruewer M, Utech M, Ivanov AI, Hopkins AM, Parkos CA, Nusrat A. 2005. Interferon-¿ induces internalization of epithelial tight junction proteins via a macropinocytosis-like process. FASEB J. 19(8):923-33


    Wang Z, Mandell KJ, Parkos CA, Mrsny RJ, Nusrat A. 2005 The Second Loop of Occludin is Required for Suppression of Raf1-induced Tumor Growth. Oncogene. 24(27):4412-20.


    Zen K, Liu Y, McCall IC, Wu T, Lee W, Babbin BA, Nusrat A, Parkos CA. 2005. Neutrophil Migration across Tight Junctions Is Mediated by Adhesive Interactions between Epithelial CAR and a JAM-Like Protein on Neutrophils. Mol. Biol. Cell, 16:2694-703


    Ivanov AI, Hunt D, Utech M, Nusrat A, Parkos CA. 2005. Differential Roles for Actin Polymerization and a Myosin II Motor in Assembly of the Epithelial Apical Junctional Complex. Mol. Bio. Cell 16(6):2636-50


    Baniulis D, Burritt JB, Taylor RM, Dinauer MC, Heyworth PG, Parkos CA, Magnusson KE, Jesaitis AJ. 2005. Monoclonal antibody CL5 recognizes the amino terminal domain of human phagocyte flavocytochrome b558 large subunit, gp91phox. Eur J Haematol. 74(4):337-47.


    Tong Q, Vassilieva EV, Ivanov AI, Wang Z, Brown GT, Parkos CA, Nusrat A. 2005 Interferon-g inhibits T84 epithelial cell migration by redirecting transcytosis of b1 integrin from the migrating leading edge. J Immunol. 175(6):4030-8.


    Liu Y, Soto I, Tong Q, Chin A, Buhring HJ, Wu T, Zen K, Parkos CA. 2005 SIRPbeta1 is expressed as a disulfide linked homodimer in leukocytes and positively regulates neutrophil transepithelial migration. J. Biol. Chem. 280(43):36132-40


    Frick C, Odermatt A, Zen K, Mandell KJ, Edens HA, Portmann R, Mazzucchelli L, Jaye DL, Parkos CA. 2005 Interaction of ICAM-1 with b2 Integrin CD11c/CD18: Characterization of a Peptide Ligand that Mimics a Putative Binding Site on ICAM-1. Eur. J. Immunol. 35: 3610-21.


    Van den Berg TK, van Beek EM, Buhring HJ, Colonna M, Hamaguchi M, Howard CJ, Kasuga M, Liu Y, Matozaki T, Neel BG, Parkos CA, Sano S, Vignery A, Vivier E, Wright M, Zawatzky R, Barclay AN. 2005. A nomenclature for signal regulatory protein family members. J. Immunol. 17: 7788-89


    Utech M, Ivanov AI, Samarin SN, Bruewer M, Turner JR, Mrsny RJ, Parkos CA, Nusrat A. 2005. Mechanism of ifn-gamma-induced endocytosis of tight junction proteins: myosin II-dependent vacuolarization of the apical plasma membrane. Mol Biol Cell. 16(10):5040-52.


    Chung E, Cook PW, Parkos CA, Park YK, Pittelkow MR, Coffey RJ. 2005 Amphiregulin causes functional downregulation of adherens junctions in psoriasis. J. Invest. Dermatol. 124(6):1134-40.


    Mandell KJ, Babbin BA, Nusrat A, Parkos CA. 2005. JAM1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity. J. Biol. Chem., 280(12):11665-74.


    Shaw SK, Ma S, Kim MB, Rao RM, Hartman CU, Froio RM, Yamg L, Jones T, Liu Y, Nusrat A, Parkos CA, Luscinskas FW. 2004 Coordinated redistribution of leukocyte LFA-1 and endothelial ICAM-1 accompany neutrophil transmigration. J. Exp. Med. 200:1571-80


    Jaye DL, Geigerman CM, Fuller RE, Akyldiz A, Parkos CA. 2004. Direct fluorochrome labeling of phage display library clones for studying binding specificities: Applications for flow cytometry and fluorescence microscopy. J. Immunological Methods, 295: 119-127


    Zen K, Utech M, Liu Y, Soto I, Nusrat A, Parkos CA. 2004. Association of BAP31 with CD11b/CD18: potential role in intracellular trafficking of CD11b/CD18 in neutrophils. J Biol Chem. 279: 44924-44930.


    Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA. 2004. JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration. Mol Biol Cell. 15 (8):3926-37.


    Ivanov AI, McCall I, Parkos CA, Nusrat A. 2004. Role for Actin Filament Turnover and a Myosin II Motor in Cytoskeleton-Driven Disassembly of the Epithelial Apical Junctional Complex. Mol Biol Cell. 15(6):2639-51.


    Mandell KJ, McCall I, Parkos CA. 2004. Involvement of the homodimer interface of junctional adhesion molecule-1 (JAM1) in epithelial barrier function. J.Biol. Chem. 279(16):16254-62


    Liu Y, O'Connor MB, Mandell KJ, Zen K, Ullrich A, B¿hing H, Parkos CA. 2004. Peptide-mediated inhibition of neutrophil transmigration by blocking CD47 interactions with SIRP alpha. J. Immunol, 172:2578-2585.


    Ivanov AI, Nusrat A, Parkos CA. 2004. Endocytosis of epithelial apical junctional proteins by a clathrin mediated pathway into a unique storage compartment. Molec. Biol. Cell. 15(1): 176-88.


    Taylor RM, Burritt JB, Baniulis D, Foubert TR, Lord CI, Dinauer MC, Parkos CA, Jesaitis AJ. 2004. Site-Specific Inhibitors of NADPH Oxidase Activity and Structural Probes of Flavocytochrome b: Characterization of Six Monoclonal Antibodies to the p22phox Subunit. J Immunol. 173(12):7349-57.


    Jaye DL, Nolte FS, Mazzucchelli L, Geigerman C, Akyildiz A, Parkos CA. 2003. Use of real time polymerase chain reaction to identify cell and tissue type selective peptides by phage display. Am J Pathol. 162(5): 1419-1429.


    Naik MU, Mousa SA, Parkos CA, Naik UP. 2003. Signaling through JAM-1 and avb3 is required for the angiogenic action of bFGF: dissociation of the JAM-1 and avb3 complex. Blood. 102: 2108 - 2114


    Bruewer M, Luegering A, Kucharzik T, Parkos CA, Madara JL, Hopkins AM, Nusrat A. 2003. Pro-inflammatory cytokines disrupt epithelial barrier function by apoptosis- independent mechanisms. J. Immunol. 171(11): 6164-72.


    Burritt JB, Foubert TR, Baniulis D, McDonald CL, Taylor RM, Mills JS, Roos D, Parkos CA, Jesaitis AJ. 2003. Functional NADPH-Oxidase epitope on human neutrophil flavocytochrome b558. J. Immunol. 170(12):6082-9


    Lawrence DW, Bruyninckx WJ, Louis NA, Lublin DM, Stahl GL, Parkos CA, and Colgan SP: 2003 Anti-adhesive role of apical decay-accelerating factor (DAF, CD55) in human neutrophil transmigration across mucosal epithelia. J. Exp. Med., 198:999-1010.


    Zen K, Liu Y, Cairo D, Parkos CA. 2002. CD11b/CD18-dependent interactions of neutrophils with intestinal epithelium are mediated by fucosylated epithelial cell surface proteoglycans. J. Immunol. 169:5270-5278


    Edens HA, Levi B, Jaye DL, Walsh SV, Reaves TA, Turner JR, Nusrat A, Parkos CA. 2002. Neutrophil interactions with intestinal epithelium: Evidence for sequential, contact- dependent effects on epithelial function by transmigrating neutrophils. J. Immunol. 169:476- 86


    Liu Y, Buhring H, Zen K, Burst SL, Schnell FJ, Williams IR, Parkos CA. 2002. Signal regulatory protein (SIRPa), regulates neutrophil transmigration. J. Biol. Chem. 277: 10028- 10036 α


    Kucharzik T, Walsh SV, Chen J, Parkos CA, Nusrat A. 2001. Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins. Am J Pathol. 159(6):2001-9


    Reaves, TA, Colgan, SP, Selvaraj, P, Walsh, S, Nusrat, A, Liang, TW, Pochet, MM, Madara, JL and Parkos, CA. 2001. Neutrophil transepithelial migration: Regulation at the apical epithelial surface by Fc-mediated events. Am. J. Physiol 280:G746-G754.


    Nusrat A, VonEichel-Streiber C, Madara JL, Verkade P, and Parkos CA. 2001. Disruption of tight junction structure and function by C. Difficile Toxins. Infect. and Immun. 69:1329- 1336.


    Barton ES, Forrest JC, Connolly JL, Chappel JD, Schnell FJ, Liu Y, Nusrat A, Parkos CA, Dermody TS. 2001. Junction adhesion molecule is a receptor for reovirus. Cell. 104:441- 451.


    Jaye, DL, Edens, HA, Mazzucchelli, LM, Parkos, CA. Novel G-protein coupled responses in leukocytes elicited by a chemotactic bacteriophage displaying a cell-type selective binding peptide. J. Immunol. 2001; 166: 7250-7259


    Walsh SV, Hopkins AN, Chen J, Narumiya S, Parkos CA, Nusrat A. 2001 Rho-Kinase regulates Tight Junction function and is necessary For Tight Junction assembly in polarized intestinal epithelia. Gastroenterology. 121(3):566-79.


    Shaw SK, Perkins BN, Lim Yaw-Chyn, Liu Y, Nusrat A, Schnell FJ, Parkos CA, Luscinskas FW. 2001. Reduced expression of Junctional adhesion molecule (JAM) and PECAM- 1(CD31) at human vascular endothelial junctions by cytokines TNFa plus IFNg does not reduce leukocyte transmigration under flow. Am J Pathol. 159(6):2281-91¿¿¿¿


    Liu Y, Merlin D, Schnell FJ, Pochet M, Madara JL, Parkos CA. 2001. The role of CD47 in neutrophil transmigration: Increased rate of migration correlates with increased cell surface expression of CD47. J Biol Chem. 276(43):40156-66.


    Kucharzik T, Walsh SV, Chen J, Parkos CA, Nusrat A. 2001. Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins. Am J Pathol. 159(6):2001-9


    Quinn MT, Swain SD, Parkos CA, Jutila KL, Siemsen DW, Jesaitis AJ, and Jutila MA. 2001. A ¿2-integrin carbohydrate neoepitope that is up-regulated on human mononuclear leukocytes by neuraminidase treatment or by cellular activation. Immunology. 104(2):185- 97.


    Nusrat A, Parkos CA, Verkade, P, Foley CS, Strohmeier GS, Liang TW, Whitehouse WI, Eastburn KK, and Madara JL. 2000. Tight junctions are membrane microdomains. J. Cell Sci. 113: 1771-1781.


    Crutchfield KL, Campbell CJ, Parkos CA, Allport JR, Camphausen RT, Goetz DJ. 2000. CD11b/CD18 coated microspheres attach to E-selectin under flow. J. Leukocyte Biol. 67:196-205.


    Liu Y, Nusrat A, Schnell FJ, Walsh S, Reaves TA, Pochet M, Foley C, and Parkos CA. 2000. Human junction adhesion molecule regulates tight junction resealing in epithelia. J. Cell Sci. 113:2363-2374.


    Mazzucchelli L, Burritt JB, Jesaitis AJ, Nusrat A, Liang TW, Schnell FJ, Parkos CA. 1999. Cell specific peptide binding by human neutrophils. Blood 93:1738-1748.


    Edens HA, Parkos CA, Liang TW, Jesaitis AJ, Cutler JE and Miettinen HM. 1999. Non- serum dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to formyl peptide receptor on neutrophils and an unknown receptor on macrophages. Infec. and Immun. 67:1063-1071.


    Friedman GB, Taylor CT, Parkos CA, Colgan SP. 1998. Epithelial permeability induced by neutrophil transmigration is potentiated by hypoxia: role of intracellular cAMP. J. Cell Physiol. 176:76-84.


    Balsam LB, Liang TW, Parkos CA. 1998. Functional mapping of CD11b/CD18 epitopes important in neutrophil-epithelial interactions: A central role of the I domain. J. Immunol. 160:5058-5065.


    Shaw S, Hermanowski-Vosatka A, Shibahara T, McCormick BA, Parkos CA, Carlson SL, Ebert EC, Brenner MB, Madara JL. 1998. Migration of intestinal intra-epithelial lymphocytes into a polarized epithelial monolayer. Am. J. Physiol. 275 (3 Pt 1):G584-G591


    Mukherjee G, Rasmussen B, Linner JG, Quinn MT, Parkos CA, Magnusson KE, and Jesaitis AJ. 1998. Organization and mobility of CD11b/CD18 and targeting of superoxide on the surface of degranulated neutrophils. Arch. Biochem. Biophys. 357:164-172.


    McCormick BA, Parkos CA, Colgan SP, Carnes DK, Madara JL. 1998. Apical secretion of a pathogen-elicited epithelial chemokine (PEEC) activity in response to surface colonization of intestinal epithelia by Salmonella typhimurium. J. Immunol. 160:455-466.


    Colgan SP, Dzus AL, Parkos CA.1996. Epithelial exposure to hypoxia modulates neutrophil transepithelial migration. J. Exp. Med. 184:1003-1015.


    Parkos CA, Colgan SP, Diamond MS, Nusrat A, Liang T, Springer TA, Madara JL. 1996. Expression and polarization of ICAM-1 on intestinal epithelia: Consequences for CD11b/CD18-mediated interactions with neutrophils. Molec. Med. 2:489-505.


    Parkos CA, Colgan SP, Liang T, Nusrat A, Bacarra AE, Carnes DK, Madara JL. 1996. CD47 mediates post-adhesive events required for neutrophil migration across polarized intestinal epithelia. J. Cell Biol. 132:437-450.


    Colgan SP, Parkos CA, McGuirk DK, Brady HR, Papayianni AA, Frendl G, Madara JL. 1995. Receptors involved in carbohydrate binding modulate intestinal epithelial-neutrophil interactions. J. Biol. Chem. 270:10531-10539.


    Parkos CA, Colgan SP, Bacarra AE, Nusrat A, Delp-Archer C, Carlson S, Su HCD, Madara JL. 1995. Intestinal epithelia (T84) possess basolateral ligands for CD11b/CD18 mediated neutrophil adherence. Am J Physiol. 268:C472-C479.


    Resnick MB, Colgan SP, Parkos CA, Delp-Archer C, Weller PF, Madara JL. 1995.GM-CSF primed eosinophils migrate across a model intestinal epithelium in response to a platelet- activating factor gradient. Gastroenterology 108: 409-416.


    Diamond MS, Alon R, Parkos CA, Quinn MT, Springer TA. 1995. Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD18). J. Cell Biol. 130:1473-1482.


    Nusrat A, Giry M, Turner JR, Colgan SP, Parkos CA, Carnes D, Lemichez E, Boquet P, Madara JL. 1995. Rho protein regulates tight junctions and perijunctional actin organization in polarized epithelia. Proc. Nat. Acad. Sci. USA 92:10629-10633.


    Parkos CA, Colgan SP, Madara JL. Interactions of neutrophils with epithelial cells: 1994. Lessons learned from the intestine. J. Amer. Soc. Nephrol. 5:138-152.


    Colgan SP, Resnick MB, Parkos CA, Bacarra AE, Delp-Archer C, Weller PW, Madara JL. 1994. Interleukin-4 Directly Modulates Function of a Model Intestinal Epithelia. J. Immunol. 153:2122-2129.


    Nusrat A, Parkos CA, Bacarra AE, Godowski PJ, Delp-Archer C, Rosen EM, and Madara JL. 1994. Hepatocyte Growth Factor/Scatter Factor Effect on Epithelia: Regulation of Intercellular Junctions in Transformed and Non-Transformed Cell Lines: Basolateral Polarization of the C-Met Receptor and Induction of Rapid Wound Repair in a Transformed Model Epithelium. J. Clin. Invest. 93:2056-2065.


    Colgan SP, Parkos CA, Matthews JB, Awtrey CS, Lichtman A, Delp-Archer C, Madara JL. 1994. Interferon-¿ induces a cell surface phenotype switch in intestinal epithelia: Down regulation of ion transport function and upregulation of immune accessory ligands. Am. J. Physiol. 267:C402-410.


    Colgan SP, Parkos CA, Delp C, Arnaout MA, Madara JL. 1993. Neutrophil migration across cultured intestinal epithelial monolayers is modulated by epithelial exposure to IFN-gamma in a highly polarized fashion. J Cell Biol. 120: 785-798.


    Madara JL, Patapoff TW, Gillece-Castro B, Colgan SP, Parkos CA, Delp C, and Mrsny RJ. 1993. 5'AMP is the neutrophil derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers. J. Clin. Invest. 91:2320-2325.


    Kaoutzani P, Parkos CA, Delp-Archer C, and Madara JL. 1993. Isolation of plasma membrane fractions from the intestinal epithelial model T84. Am. J. Physiol. 1993; 264:C1327-1335.


    Colgan SP, Serhan CN, Parkos CA, Delp-Archer C, Madara JL. 1993. Lipoxin A4 modulates transmigration of human neutrophils across intestinal epithelial monolayers. J. Clin. Invest.92:75-82.


    Madara JL, Colgan SP, Nusrat A, Delp C, Parkos CA. 1992. A simple approach to measurement of electrical parameters of cultured epithelial monolayers: Use in assessing neutrophil-epithelial interactions. J. Tiss. Cult. Meth. 14:209-216.


    Parkos CA, Colgan SP, Delp C, Arnaout MA, Madara JL. 1992. Neutrophil migration across a cultured epithelial monolayer elicits a biphasic resistance response representing sequential effects on transcellular and paracellular pathways. J. Cell Biol. 117:757-764.


    Colgan SP, Parkos CA, Delp C, Awtrey CS, Matthews JB, Madara JL. 1992. The human promyelocytic leukemia cell line HL-60 produces a factor which causes chloride secretion in cultured epithelial cells. J Leuk Biol 52:183-187.


    Quinn MT, Curnutte JT, Parkos CA, Mullen ML, Scott PJ, Erikson RW, Jesaitis AJ. 1992. Reconstitution of defective respiratory burst activity with partially purified human neutrophil cytochrome b in two genetic forms of chronic granulomatous disease: possible role of rap1A. Blood 79:2438-2445.


    Madara JL, Parkos CA, Colgan SP, MacLeod RJ, Nash S, Matthews J, Delp C, Lencer W. 1992. Chloride secretion in a model intestinal epithelium induced by a neutrophil derived secretagogue. J Clin Invest. 89:1938-1944.


    Parkos CA, Delp C, Arnaout MA, Madara JL. 1991. Neutrophil migration across a cultured intestinal epithelium: Dependence on a CD11b/CD18 mediated event and enhanced efficiency in physiologic direction. J. Clin. Invest. 88:1605-1612.


    Nash S, Parkos C, Nusrat A, Delp C, Madara JL. 1991. In vitro model of intestinal crypt abscess: A novel neutrophil-derived secretagogue activity. J. Clin. Invest. 87:1474-1477


    Jesaitis AJ, Buescher ES, Harrison D, Quinn MT, Parkos CA, Livesey S, Linner J. 1990. Ultrastructural localization of cytochrome b in the membranes of resting and phagocytosing human granulocytes. J. Clin. Invest. 85:821-835.


    Quinn MT, Parkos CA, Walker L, Orkin SH, Dinauer MC, Jesaitis AJ. 1989. Association of a RAS-related protein with cytochrome b of human neutrophils. Nature 342:198-200.


    Quinn MT, Parkos CA, Jesaitis AJ. 1989. The lateral organization of components of the membrane skeleton and superoxide generation in the plasma membrane of stimulated human neutrophils. Biochim. Biophys. Acta 987:83-94.


    Parkos CA, Dinauer MC, Jesaitis AJ, Orkin SH, Curnutte JT. 1989. Absence of both the 91kD and 22kD subunits of human neutrophil cytochrome b in two genetic forms of chronic granulomatous disease. Blood 73:1416-1420.


    Bokoch GM, Parkos CA, Mumby SM. 1988. Purification and characterization of the 22000-Dalton GTP binding protein substrate for ADP-ribosylation by botulinum toxin,G22k. J. Biol. Chem. 263:16744-16749.


    Parkos CA, Allen RA, Cochrane CG, Jesaitis AJ. 1988. The quaternary structure of the plasma membrane b-type cytochrome of human granulocytes. Biochim. Biophys. Acta. 932:71-83.


    Parkos CA, Dinauer MC, Walker LE, Allen RA, Jesaitis AJ, Orkin SH. 1988. The primary structure and unique expression of the 22kD light chain of human neutrophil cytochrome b. Proc. Nat. Acad. Sci. USA 85:3319-3323.


    Bokoch GM, Parkos CA. 1988. Identification of novel GTP binding proteins in the human neutrophil. Febs Lett. 227:66-70.


    Parkos CA, Wahrenbrock EA. 1987. Acute effects of hypercapnia and hypoxia on minute ventilation in unrestrained weddel seals. Respiration Physiology. 67:197-207.


    Parkos CA, Allen RA, Cochrane CG, Jesaitis AJ. 1987. Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides of Mr=91000 and Mr=22000. J. Clin. Invest. 80:732-742.


    Omann GM, Swann WN, Oades ZG, Parkos CA, Jesaitis AJ, Sklar LS. 1987. N-formylpeptide receptor dynamics, cytoskeletal activation, and intracellular calcium response in human neutrophil cytoplasts. J. Immunol. 139:3447-3445.


    Dinauer MC, Orkin SH, Jesaitis AJ, Parkos CA. 1987. The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil b-cytochrome complex. Nature 327:717-721.


    Parkos, CA. 1987. Investigations on the function and structure of a b-type cytochrome expressed in the plasma membrane of human granulocytes. Ph.D. Thesis.


    Parkos CA, Cochrane CG, Schmitt M, Jesaitis A.J. 1985. Regulation of the oxidative response of human granulocytes to chemoattractants: no evidence for stimulated traffic of redox enzymes between endo and plasma membranes. J. Biol. Chem. 260:6541-6547.


    McCormick BA, Nusrat A, Parkos CA, D'Andrea L, Hofman PM, Carnes D, Liang, TW, Madara JL. 1997. Unmasking of intestinal epithelial lateral membrane ¿1 integrin consequent to transepithelial neutrophil migration in vitro facilitates inv-mediated invasion by yersinia. Infec. and Immun. 65:1414-1421.


    Other Publications -

    Chin AK, Parkos CA. 2007. Pathobiology of neutrophil transepithelial migration: Implications in mediating epithelial injury. Annual Reviews in Pathology, 2:111-44.


    Severson EA, Parkos CA. 2007. Structure and Function of JAM proteins. Adhesion Molecules: Function and Inhibition (PIR) In Press.


    Chin AK, Parkos CA. 2006. Neutrophil transepithelial migration and epithelial barrier function in IBD- Potential targets for inhibiting neutrophil trafficking. Ann. New York Acad. of Sciences, 1072:276-287


    Lee WY, Chin AK, Voss S, Parkos CA. In vitro neutrophil transepithelial migration. In Methods in Cell Biology. In press 2006.


    Ivanov AI, Nusrat A, Parkos CA. 2005. Endocytosis of the apical junction complex: Mechanisms and possible roles in regulation of epithelial function. Bioessays. Apr;27(4):356-65.


    Mandell KJ, Parkos CA. 2005. The JAM family of proteins. Adv. Drug Del. Rev. 57(6):857-67.


    Ivanov AI, Nusrat A, Parkos CA. 2005. The epithelium in inflammatory bowel disease: Potential role of endocytosis of junctional proteins in barrier disruption. Novartis Found. Symp. 263:115-24; discussion 124-32, 211-8.


    Reaves TA, Chin AC, Parkos CA. 2005. Neutrophil transepithelial migration: Role of Toll- like receptors in mucosal inflammation. Memorias do Instituto Oswaldo Cruz. 100 (Suppl 1): 191-198.


    Liu Y, Shaw SK, Ma S, Luscinskas FW, Parkos CA. 2004, Regulation of leukocyte transmigration: cell surface interactions and signaling events. J. Immunol. 172(1): 7-13


    Zen K and Parkos CA. 2003. Leukocyte epithelial interactions. Curr. Opin. Cell Biol. 15:557-564.


    Edens HA, Parkos CA. 2003. Neutrophil Transendothelial Migration and Alteration in Vascular Permeability; Focus on Neutrophil-derived Azurocidin. Seminars in Hematology. 10(1):25-30.


    Luscinskas FW, Ma S, Nusrat A, Parkos CA, Shaw SK. 2002. The role of endothelial cell lateral junctions during leukocyte trafficking. Immunol Rev. 2002 Aug;186:57-67.


    Luscinskas FW, Ma S, Nusrat A, Parkos CA, and Shaw SK. 2002. Leukocyte transendothelial migration: A junctional affair. Semin Immunol. 14:105-13.


    Jaye DL and Parkos CA. 2000. Neutrophil migration across intestinal epithelium. Ann. New York Acad. of Sciences. 915:151-161.


    Madara JL, Colgan SP, McCormack BA, Strohmeier G, Parkos, CA. 1997. Pathobiology of neutrophil-epithelial interactions. In Pathogenesis of Inflammatory Bowel Disease. FalkSymposia. Edens HA, and Parkos CA. 2000. Modulation of epithelial and endothelial paracellular permeability by leukocytes. Adv. Drug Del. Rev. 41:315-328.


    Parkos CA. 1997. The molecular basis of neutrophil transepithelial migration. Bio Essays, 19:865-873.


    Parkos CA. 1997. Neutrophil adhesive interactions with intestinal epithelial cells. Am. J. Physiol. (Gastrointest. Liver Physiol. 36) 273:G763-G768.


    Colgan SP, Parkos CA, Bacarra AE, Madara JL. 1995. Influence Of Myeloid Blind Panel Antibodies on Neutrophil (PMN) Transmigration Across Intestinal Epithelial Monolayers. In: Leucocyte Typing V: White cell differentiation antigens. SF Schlossman, L Boumsell, W Gilks, JM Harlan, T Kishimoto, J Ritz, S Shaw, RL Silverstein, TA Springer, TF Tedder, RF Todd (eds). Oxford Press, London, UK.


    Quinn MT, Parkos CA, Jesaitis AJ. 1995. Purification of human neutrophil NADPH oxidase cytochrome b558 and association with Rap1A. Methods Enzymology, 255:476-487.


    Madara JL, Parkos CA, Colgan SP, Nusrat A, Atisook K, Kaoutzani P. 1992. The movement of solutes across tight junctions. New York Academy of Sciences, 664:47-60.


    Parkos CA, Quinn M, Sheets S, Jesaitis AJ. 1992. The Structure of the Human Neutrophil Plasma Membrane b-Type Cytochrome Involved in Superoxide Production. In: Jesaitis AJ, Dratz EA, eds. The Molecular Basis of Oxidative Damage by Leukocytes. Boca Raton, CRC Press Inc.


    Madara JL, Nash S, Parkos C. 1992. Neutrophil-Epithelial Cell Interactions in the Intestine. In: Wong P, Serhan C, eds. Cell-Cell Interactions in the Release of Inflammatory Mediators. Plenum Press, New York.