Pathology Faculty Member

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    Experimental Pathology
    105-L Whitehead Bldg., 615 Michael St.
    Atlanta, GA 30322
    404 727-8545 Phone

    For an expert Pathology consultation,
    or to send a specimen, call:

    1-404-712-5947


    Andrew Scott Neish, MD

    Epithelial Pathobiology
    Immunology
    Microbial Pathogenesis

    Professor
    Pathology & Laboratory Medicine


    Pathology Faculty Photo

    Pathology Division(s):
    Anatomic Pathology
    Experimental Pathology


    Email Address:
    aneish@emory.edu


    Education:

    B.A.   Wake Forest University, Winston-Salem, NC, - 1983

    M.D.   University of North Carolina School of Medicine, Chapel Hill, NC, - 1988



    Research Interests:

    - Host-microbe interactions
    Host-microbe interactions: Our laboratory studies innate immunity and epithelial biology relevant to normal intestinal physiology as well as infectious (Gram negative enterocolitis), idiopathic (idiopathic inflammatory bowel disease) and developmental (necrotizing enterocolitis) inflammatory disorders of the gut. Specifically, our research program investigates the interactions of enteric pathogens and members of the normal gut microbiota and their products with epithelial cells in an effort to understand the molecular mechanisms of pathological and commensal eukaryotic-prokaryotic relationships. Eukaryotic cells recognize bacteria via surface receptors (pattern recognition receptors) that bind conserved microbial structural motifs. This includes the formyl peptide receptors, which can mediate both inflammatory and non inflammatory signaling, and potentially play key roles in normal intestinal homeostasis and response to infection. These receptors induce the formation of reactive oxygen species, which can play a wide role in host homeostatic signaling and maintenance of normal physiological functions. We are actively exploring how beneficial commensal bacteria modulate these processes. Additionally, pathogenic bacteria are thought to mediate interactions with eukaryotic cells by preformed effector proteins that are translocated into the host epithelia and usurp normal cellular functions. We are also interested in exploiting these proteins as potential therapeutics. The laboratory employs a variety of microbiologic, genetic, biochemical and cell biological techniques to approach theses questions, including use of mammalian cell culture, murine and Drosophila models and large-scale informatics approaches.


    -
    Dr. Neish's research focuses on the interactions of bacteria with human epithelial cells in an effort to understand the molecular mechanisms of immune and inflammatory reactions that may mediate pathogenic and commensal relationships.



    Honors / Awards:

    Fellow of the American Gastroenterological Association, 2011

    American Association of University Pathologists, 2009

    Burroughs Wellcome Investigator in Pathogenesis of Infectious Disease, 2004-2009

    Dean's Clinical Investigator Award, Emory University, 2001-2006

    Albert E. Levy Science Research Award, Emory University,, 2001

    Bugher Foundation Fellow, Program in Molecular Biology of the Cardiovascular System, 1993-1994

    Merit Award for Academic Achievement, University of North Carolina School of Medicine, Chapel Hill, NC, 1984-1986

    Cum Laude, Wake Forest University, Winston-Salem, NC, 1983

    Beta Beta Beta, Wake Forest University, Winston-Salem, NC, 1982

    Specialty Boards:

    1993  Anatomic Pathology


    Selected Publications:

    Published and Accepted Research Articles -

    Leoni G, Alam M, Neumann P-A, Lambeth JD, Cheng G, McCoy J, Hilgarth RS, Kundu K, Murthy N, Kusters D, Reutelingsperger C, Perretti M, Parkos CA, Nusrat A1, Neish AS1 Co-senior authors. Annexin A1- FPR1-Nox1 dependent redox signaling promotes epithelial wound repair. J Clin Invest, 2013; 123(1):443-54


    Wu H, Jones RM, Neish AS. The Salmonella effector AvrA mediates bacterial intracellular survival during infection in vivo. Cell Micro, 2012; 14:28-39.


    Jones RM, Sloane V, Wu H, Luo L, Kumar A, Vijay-Kumar M, Gewirtz AT, Neish AS Flagellin administration protects gut mucosa tissue from irradiation induced apoptosis via MKP-7 activity. Gut; 2011 60:648-57


    Wentworth CC, Alam A, Jones RM, Nusrat A, Neish AS. Enteric commensal bacteria induced ERK via FPR dependant redox modulation of DUSP3. J. Biol. Chem, 2011; 286:38448-38455.


    Swanson P , Kumar A, Samarin S, Vijay-Kumar M, Kundu K, Murthy N, Hansen J, Nusrat A, Neish AS. Enteric commensal bacteria potentiates epithelial restitution via ROS-mediated inactivation of FAK phosphatases. Proc. Natl. Acad. Sci. 2011;108:8803-8


    Wentworth CC, Jones RM, Kwon Y-M, Nusrat A, Neish AS, Commensal-Epithelial Signaling Mediated via Formyl Peptide Receptors. A J. Path, 2010; 177:2782-90.


    Lin PW, Myers LS, Ray L, Song S-C, Nasr TR, Berardinelli AJ, Kundu K, Murthy N, Jason JM, Neish AS. Lactobacillus rhamnosus blocks inflammatory signaling in vivo via reactive oxygen species generation. Free Rad Biol& Med, 2009; 47:1205-11.


    Kumar A, Wu H, Collier-Hyams LS, Kwon YM, Hansen JM, Neish AS. The bacterial fermentation product butyrate influences epithelial signaling via ROS mediated changes in Cullin-1 neddylation. J. Immunol. 2009;182:538-46.


    Fournier B, Gewirtz AT, Williams IR, Neish AS, TLR5-dependent regulation of inflammation in systemic Salmonella enterica serovar Typhimurium infection. Infect Immune, 2009, 77:4121-29.


    Jones R, Wu H, Wentworth C, Luo L, Collier-Hyams L, Neish AS. Salmonella AvrA coordinates suppression of host immune and apoptotic defenses via JNK pathway blockade. Cell Host Microbe, 2008; 3:233-44.


    Lin PW, Nasr TR, Berardinelli AJ, Kumar A, Neish AS. The probiotic Lactobacillus GG may augment host defense by regulating apoptosis and promoting cytoprotective responses in the developing murine gut. Ped Research, 2008;64:511-6.


    Kumar A, Wu H, Collier-Hyams LS, Hansen JM, Li T, Yamoah K, Pan Z-Q, Jones DP, Neish AS. Commensal bacteria repress cullin dependant-signaling via generation of reactive oxygen species. EMBO J. 2007;26:4457-66.


    Yu Y, Nagai S, Wu H, Neish AS, Koyasu S, Gewirtz AT.TLR5-mediated PI3K activation negatively regulates flagellin-induced pro-inflammatory gene expression. J. Immunol. 2006; 176:6194-201.


    Ghartey-Tagoe EB, Babbin BA, Nusrat A, Neish AS, Prausnitz MR. Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation. Int. J. Pharmaceutics. 2006; 315:122-133.


    Zeng H, Wu H, Jones R, Yu Y, Lin P, Gewirtz AT, Neish AS. Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. Am. J. Physiol. 2006; 290: G96-G108.


    Vijay-Kumar M, Wu H, Jones R, Grant G, Babbin B, King TP, Kelly D, Gewirtz AT, Neish AS. Flagellin suppresses epithelial apoptosis and limits disease during enteric infection. Am. J. Path. 2006; 169:1686-1700.


    Collier-Hyams LS, Batten B, Sloane V, Neish AS. Bacterial modulation of epithelial signaling via deneddylation of Cul-1. J. Immunol (Cutting edge). 2005; 175:4194-4198.


    Franco AT, Israel DA, Washington MK, Fox JG, Rogers AB, Neish AS, Collier-Hyams L, Guillermo Perez-Perez GI, Hatakeyama M, Whitehead R, Krishna U, Ferrero RL, Gaus K, O'Brien DP, Peek RM. Activation of ¿-catenin by carcinogenic Helicobacter pylori. Proc. Natl. Acad. Sci. 2005;102:10646-10651.


    Ghartey-Tagoe EB, Morgan JS, Neish AS, Prausnitz MR. Increased permeability of intestinal epithelial monolayers mediated by electroporation. J. Control Release 2005;103:177-190.


    Vijay-Kumar M, Gentsch JR, Kaiser WJ, Borregaard H, Offerman MK, Neish AS, Gewirtz AT. Protein kinase R mediates intestinal epithelial gene remodeling in response to double stranded RNA and live rotavirus. J. Immunol 2005; 174: 6322-6331.


    Lin PW, Simon PO, Gewirtz AT, Selsted ME, Neish AS, Ouellette AJ, Madara JL, Lencer WI. Paneth cell cryptdins act in vitro as apical paracrine regulators of the innate inflammatory response. J. Biol.Chem. 2004; 297:19902-19907.


    Sun J, Hobert ME, Rao AS, Neish AS, Madara JL. Bacterial activation of beta-catenin signaling in human epithelia. Am J Physiol Gastrointest Liver Physiol. 2004


    Ghartey-Tagoe EB, Morgan JS, Ahmed K, Neish AS, Prausnitz MR. Electroporation-mediated delivery of molecules to model intestinal epithelia. Int. J. Pharmeceutics, 2004; 270:127-138.


    Yu Y, Zeng H, Vijay-Kumar M, Neish AS, Merlin D, Sitaraman SV, Gewirtz AT. STAT signaling underlies difference between flagellin-induced and TNF alpha -induced epithelial gene expression. J Biol Chem. 2004; 279:35210-35218.


    Yu Y, Zeng H, Lyons S, Carlson A, Merlin D, Neish AS, Gewirtz AT. TLR5-mediated activation of p38 MAPK regulates epithelial IL-8 expression via a post-transcriptional mechanism. Am. J. Physiol. Gastrointest. Liver Physiol. 2003; 285:G282-G290.


    Zeng H, Carlson AQ, Guo Y, Yu Y, Collier-Hyams L, Madara JL, Gewirtz AT, Neish AS, Flagellin is the major proinflammatory determinants of enteropathogenic Salmonella. J. Immunol. 2003; 171:3668-3574.


    Hunter S, Young A, Olson J, Brat DJ, Bowers G, Wilcox JN, Jaye D, Mendrinos S, Neish AS. Differential expression between pilocytic and anaplastic astrocytomas: identification of apolipoprotein D as a marker for low-grade, non-infiltrating primary CNS neoplasms. J. Neuropathol. Exp. Neurol. 2002; 61:275-281.


    Collier-Hyams LS, Zeng H, Sun J, Tomlinson AD, Bao ZQ, Chen H, Madara JL, Orth K, Neish AS. Salmonella AvrA effector inhibits the key proinflammatory, anti-apoptotic NF-kB pathway. J. Immunol (Cutting edge). 2002; 169: 2846-2850.


    Gewirtz AT, Collier-Hyams LS, Young AN, Kucharzik T, Guilford W, Parkinson J, Williams IR, Neish AS, Madara JL. Lipoxin A4 analogs attenuate induction of intestinal epithelial pro-inflammatory gene expression and reduce the severity of DSS-induced colitis. J. Immunol. 2002; 168: 5260-5267.


    Gewirtz AT, Collier-Hyams LS, Young AN, Kucharzik T, Guilford W, Parkinson J, Williams IR, Neish AS, Madara JL. Lipoxin A4 analogs attenuate induction of intestinal epithelial pro-inflammatory gene expression and reduce the severity of DSS-induced colitis. J. Immunol. 2002; 168: 5260-5267.


    Young AN, Lim S, Cohen C, Marshall F, Petros J, Amin M, Neish AS. Expression profiling of renal epithelial neoplasms: a method for tumor classification and discovery of diagnostic molecular markers. Am. J. Path. 2001; 158:1639-1651.


    Gewirtz AT, Simon PO, Schmitt CK, O'Brian A, Neish AS, Madara JL. S. typhimurium translocates flagellin across model intestinal epithelia where it acts as an essential pro-inflammatory factor. J. Clin. Invest. 2001; 107:99-109.


    Evans C-O, Young AN, Brown MR, Brat DJ, Parks JS, Neish AS, Oyesiku NM. Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarray analysis and quantitative reverse transcription-polymerase chain reaction. J. Clin. Endo. Metab. 2001; 86:3097-3107.


    Gewirtz A, Rao A, Simon PO, Merlin D, Carnes D, Madara JL, Neish AS. S. typhimurium induces epithelial IL-8 expression via Ca mediated activation of the NF-kB pathway, J. Clin. Invest. 2000; 105: 79-92.


    Neish AS, Gewirtz AT, Zeng H, Young AN, Hobert M, Karmali V, Rao AS, Madara JL. Prokaryotic regulation of epithelial responses by inhibition of IkB-a ubiquitination, Science. 2000; 289, 1560-1563.


    Neish AS, Anderson SF, Parvin JD. Factors associated with the mammalian RNA polymerase II holoenzyme. Nuc. Acids Res. 1998; 26: 847-853.


    Gewirtz A, McCormick B, Petasis NA, Gronert K, Neish AS, Serhan CN, Madara JL. Pathogen-induced chemokine secretion from model intestinal epithelium is inhibited by lipoxin A4 analogs, J. Clin. Invest. 1998; 101:1860-1869.


    Gerritsen ME, Williams A, Neish AS, Moore S, Shi Y, Collins T. CBP/p300 are transcriptional coactivators of p65. Proc. Natl. Acad. Sci. USA.


    Read MA, Neish AS, Gerritsen ME, Collins T. Nuclear IkB-a and the post-induction transcriptional repression of E-selectin and VCAM1. J. Immunol.


    Read MA, Neish AS, Luscinskas FW, Palombella VJ, Maniatis T, Collins T. The proteasome pathway is required for cytokine-induced endothelial leukocyte adhesion molecule expression.


    Neish AS, Read MA, Thanos D, Pine R, Maniatis T, Collins T. Endothelial IRF-1 cooperates with NF-kB as a transcriptional activator of vascular cell adhesion molecule-1. Mol. Cell. Biol.


    Neish AS, Khachigian LM, Park A, Baichwal VR, Collins T. Sp1 is a component of the cytokine-inducible enhancer in the promoter of vascular cell adhesion molecule-1. J. Biol. Chem.


    Shu HB, Agranoff AB, Nabel EG, Leung K, Duckett CS, Neish AS, Collins T, Nabel GU. Differential regulation of VCAM-1 gene expression by specific NF-kB subunits in endothelial and epithelial cells


    Neish AS, Williams AJ, Palmer HJ, Whitley MZ, Collins T. Functional analysis of the human vascular cell adhesion molecule-1 promote


    Neish AS, Loh E, Schoen F. Myocardial changes in cardiac transplant-associated coronary arteriosclerosis: Potential for timely diagnosis


    Ridker P, Chertow G, Karlson G, Neish AS, Schoen F. Bioprosthetic tricuspid valve stenosis associated with extensive plaque deposition in carcinoid heart disease. Am. Heart J


    Book Chapters -

    Bienenstock J, Gibson G, Klaenhammer T, Walker WA, Neish AS. New insights into probiotic mechanisms: A harvest from functional and metagenomic studies. Gut Microbes 2013;4:94-100


    Jones RM, Mercante JW, and Neish AS. Reactive oxygen production induced by the gut microbiota: Pharmacotherapeutic implications. Curr. Med. Chem. 2012;19:1519-29.


    Neish AS and Naumann M, Microbial induced immunomodulation by targeting the NF-kB system, Trends Microbiol. 2011;19:596-605.


    Eckmann L, and Neish AS, NF-kB and mucosal homeostasis. In: Karin M ed. NF-kB. In Health and Disease, 2010, Advances in Microbiology and Immunology.


    Neish AS, Microbes in gastrointestinal health and disease, Gastroenterology, 2009;136:65-80.


    Lin, P., and Neish, AS, Innate immunity and epithelial biology: Special considerations of the neonatal gut. In: Neu J. ed. Neonatology: Questions and Controversies: Gastroenterology and Nutrition, 2008, Saunders/Elsevier, 51-72.


    Neish AS. Flagellin mediated inflammation and anti-apoptosis, Themes review. Am. J. Physiol. In press


    Neish, AS. Cell Signaling pathways as targets for bacterial evasion and immunity. In: McFall-Ngai M, Henderson B, and Ruby E. eds. The influence of bacterial communities on host biology, 2005, Cambridge University Press, 375-398.


    Collier-Hyams LS and Neish AS. Immune Relationship between Commensal Flora and the Mammalian Host. Cell Mol Life Sci. 2005; 62: 1339-1348.


    Neish, AS. Cell Signaling pathways as targets for bacterial evasion and immunity. In: McFall-Ngai M, Henderson B, and Ruby E. eds. The influence of bacterial communities on host biology, 2005, Cambridge University Press, 375-398.


    Neish, AS. Bacterial inhibition of eukaryotic pro-inflammatory pathways. Immunologic Res. 2004; 29: 175-185.


    Neish, AS. Molecular aspects of intestinal epithelial cell-bacterial interactions that determine the development of intestinal inflammation. Inflamm. Bowel. Dis. 2004; 10:159-168.


    Neish, AS. Microbial interference with host inflammatory responses. In: Hecht G, ed. Microbial Pathogens and the Intestinal Epithelial Cell. 2003 ASM press, Washington DC.175-190.


    Neish AS. The gut microflora and intestinal epithelial cells: A continuing dialog. Microbes and Infection, 2002; 4: 309-317.