144 Whitehead Bldg., 615 Michael St.
Atlanta, GA 30322
404 712-2326 Phone
B.S. Univeristy of California, Los Angeles - Mathematics, 1978 - 1983
Ph.D. University of California, Los Angeles - Neurosciences, 1983 - 1988
- How bacterial and viral pathogens interface with the host
The general goal of my laboratory is to understand how bacterial and viral pathogens interface with the host. We have focused on two mechanistic aspects of this interface: (i) the immunological detection and clearance of the infection, and (ii) host systems utilized by the pathogen to facilitate infection. Our work has focused on two pathogens: enteropathogenic E.coli (and the related enterohemmorhagic E. coli, the cause of "raw hamburger disease), and vaccinia virus (a relative of variola virus, the cause of smallpox). We have utilized a combination of experimental approaches including cell biology assays based on high resolution deconvolution microscopy, biochemical systems that permit reconstitution of cellular responses with cytoplasmic extracts in permeablized cells, mouse genetic systems that model human disease, and permit investigation of the immunological response to the pathogen, and a C. elegans model system which allows genetic dissection of both host and pathogen. A long-term goal of the laboratory is to develop approaches that will permit identification of agents useful in treating disease. There is considerable impetus for developing such agents to treat infections caused by bacterial and viral pathogens: development of resistance to antibiotic or other chemotherapies looms as perhaps the single most important public health concern confronting humans in the coming century. In this regard, our current efforts have led to the development and testing of novel inhibitors of pathogenic E.coli infections, which interfere with the interface between host and pathogen but not with bacterial growth. As such, these inhibitors will not easily engender development of drug resistance
National Cancer Institute Research Fellowship, UCSF, 1993-2001
National Institutes of Health Senior Postdoctoral Fellowship, UCSF, 1991-1993
Bank of America-Giannini Foundation Fellow, UCLA, 1990-1991
Bank of America-Giannini Foundation Fellow, UCLA, 1989-1990
N.I.H. Predoctoral trainee in cell and molecular biology, UCLA, 1985-1987
A.R.C.S. Scholar (Research award), UCLA, 1983-1985
Richardson, TA, Sherman M, Kalman D, Morgan ET. Expression of UDP-glucuronosyltransferase isoform mRNAs during inflammation and infection in mouse liver and kidney. Drug Metab Dispos. 2006 Mar; 34(3):351-3.
Bommarius,B., Maxwell, D., Leung, S. Corbett, A. Bornmann, W. and Kalman, D. Enteropathogenic E.coli Tir is an SH2/3 ligand that recruits and activates tyrosine kinases required for pedestal formation. 2006. Journal of Cell Biology (submitted).
Williams, I.R., Sherman, M.A., Kalman, D. and J.M.A. Klapproth. C. rodentium LifA/efa1 is essential for colonic hyperplasia and inflammation in mice. 2004. Infection and Immunity 2005 Mar;73(3):1441-51. Erratum in: Infect Immun. 2005 May;73(5):3196.
Wei, L.C., Hilliard, A. Kalman, D. and M.A. Sherman. Mast cells are required for protection from enteric bacterial infection. Infection and Immunity 2005 73(4):1978-85).
Reeves, P. Bommarius, B, Swimm, A, Veach, D., Bornmann, WG, Sherman, M., and Kalman, D. Disabling poxvirus pathogenesis by inhibition of Abl-family tyrosine kinases. 2005 Nature Medicine. 11: 731-738 (June 26; [Epub ahead of print]).
Chahroudi, A., Garber, DA, Reeves, P., Liu, L., Kalman, D. and Feinberg, MB. Vaccinia virus and modified vaccinia virus Ankara do not replicate in dendritic cells and induce apoptosis with differential timing. 2005. J. Virology, in press.
Ayanaful. A., Dolan-Livengood, J.D., Lewis, T., DeZalia, M., Kalman, L.V., Sherman, MA, Benian, GM, and Kalman, D. Paralysis and Killing of C. elegans by enteropathogenic E. coli requires the bacterial tryptophanase gene. 2005. Molecular Microbiology. Online publication date: 5 July 2005. Mol Microbiol. 2005 Aug;57(4):988-1007.
Salazar, G. Craige, B. Love, R., Kalman, D. and Faundez, V. Vglut1 and ZnT3 co-targeting mechanisms regulate vesicular zinc stores in PC12 cells. 2005. Journal of Cell Science 118, 1911-1921.
Swimm, A. Bommarius, B, Li, Y., Reeves, P., Cheng, D. , Veach, D., M. Sherman Bornmann, WG, and Kalman, D. Enteropathogenic E.coli use redundant tyrosine kinases to form actin pedestals. Molecular Biology of the Cell. 2004. 15:3520-3529.
Sherman, M.A. and D. Kalman. 2004. Initiation and resolution of mucosal inflammation. Immunologic Research. 29:241-52.
Swimm, A., Bommarius, B., Reeves, P., Sherman, M., and Kalman, D. Complex kinase requirements for EPEC pedestal formation. 2004. Nature Cell Biology 6, 795 (Correspondence)
Srinivasan, S., Wang, F., Glavas, S., Ott., A., Hofmann, F., Aktories, K., Hahn, K. Kalman, D., and Bourne, H.R. Rac and Cdc42 Play Distinct Roles in Neutrophil Chemotaxis. Journal of Cell Biology. 2003 J. Cell Biol. 2003. 160:375-85.
Rong, R., Ahn, J-H, Huang, H., Nagata,E., Kalman, D., Kapp, J., Tu, J., Worley, P., Snyder, S., and Ye, K., 2003. PI-3 kinase enhancer-Homer complex couples mGluR1 to PI3 kinase, preventing neuronal apoptosis. Nature Neuroscience 6:1153-1161
Kuo, C., LaMontagne, K.R., Garcia-Cardena, G., Ackley, B., Kalman, D., Park, S., Christofferson, R., Kamihara, J., Ding, Y-H., Lo, K-M., Gillies, S., Folkman, J., Mulligan, R., and Javaherian, K. 2001. Oligomerization-dependent regulation of Motility and Morphogenesis by the Collagen XVIII NC1/Endostatin Domain. Journal of Cell Biology 152: 1-15.
Smith-McCune, K., Kalman, D., Robbins, C., Shivakumar, S., Yuschenkoff, L., and Bishop, J.M. (1999). Intranuclear localization of human papillomavirus 16 E7 during transformation and preferential binding of E7 to the Rb family member p130. (1999). Proceedings of the National Academy of Sciences, U.S.A. 96:6999-7004.
Kalman, D., Weiner, O.D., Goosney, D.L., Sedat, J.W., Finlay, B.B., Abo, A., and Bishop, J.M. 1999. Enteropathogenic E. coli acts through WASP and the Arp2/3 complex to form actin pedestals. Nature Cell Biology 1:389-391.
Kalman, D., Gomperts, S.N., Hardy, S., Kitamura, M., and Bishop, J.M. 1999. Ras Family GTPases control Growth of Astrocyte Processes. Molecular Biology of the Cell. 10:1665-1683.
Lissin, D.V., Gomperts, S.N., Carroll, R., Chadwick, C.W., Kalman, D., Kitamura, M., Hardy, S., Nicoll, R.A., Malenka, R.C., and von Zastrow, M. 1998. Activity differentially regulates the surface expression of synaptic AMPA and NMDA glutamate receptors. Proceedings of the National Academy of Sciences, U.S.A. 95:7097-7102
Kalman, D., Whittaker, K. Bishop, J.M., and O'Lague, P.H. 1993. Domains of E1A that bind p105Rb, p130 and p300 are required to block NGF-induced neurite growth in PC12 cells. Molecular Biology of the Cell 4: 353-361.
Kalman, D., Wong, B., Horvai, A.E., Cline, M.J. and O'Lague, P.H. 1990. Nerve growth factor acts through cAMP-dependent kinase to increase the number of sodium channels in PC12 cells. Neuron 2:355-366.
Kalman, D., O'Lague, P.H., Erxleben, C., and Armstrong, D. 1988. Calcium-dependent inactivation of dihydropyridine-sensitive calcium channels in GH3 cells. The Journal of General Physiology 92:531-548.
Trumpp, A., and Kalman, D. Science, Society and the Nobel Prize Nature Cell Biology 6, 173 (Book Review).