Profile of a Pathology Faculty Member

Pathology Faculty Photo

J. David Lambeth, MD, PhD

Cancer Biology
Cardiovascular Biology

Professor
Pathology & Laboratory Medicine

Pathology Division(s):
Experimental Pathology
Email Address:   noxdoc@mac.com

Education:

B.A.   with Highest Honors, Southern Methodist University, 1968 - 1972
PhD   in Biochemistry, Duke University, 1972 - 1976
MD   Duke University, 1976 - 1977

Research Interests:

- Reactive Oxygen Species (ROS) Studies focus on reactive oxygen species (ROS) such as superoxide and hydrogen peroxide, and their biological roles, for example, in innate immunity, mitogenic growth, apoptosis, angiogenesis, and biosynthesis/ remodelling of extracellualr matrix. Starting in 1999, we described a new family of ROS-generating enzymes, the Nox/Duox family. This group of enzymes catalyzes the regulated generation of superoxide in response to signals including hormones, growth factors and immune mediators. The enzymology and regulation of ROS generation by the Nox enzymes and their regulatory proteins is an active area of investigation in our group. The biological function of these enzymes is being investigate in various model systems including cell culture, Drosophila melanogaster, C. elegans and mouse. In addition, the function of these enzymes in pathological conditions including cancer and atherosclerosis, and in normal aging is under active investigation. These enzymes now provide novel targets for development of anti-cancer and anti-arteriosclerotic drugs, which are currently under development by our group. These are predicted to be useful in the treatment and prevention of hypertension, atherosclerosis, and certain forms of cancer. The laboratory uses multi-disciplinary approaches ranging from enzymology and biochemistry to cell biology to animal models.

Honors / Awards:

  • Named Eminent Scientist of the Year 2003 by International Research Promotion Council, 2003
  • NIH Research Career Development Award, 1982-1987
  • Elected Teacher of the Year by the Medical School Class of 1985, 1981
  • Insurance Medical Scientist Scholarship from Prudential Insurance Company, 1974-1977
  • Phi Beta Kappa, 1971

Selected/Most Recent Publications:

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Published and Accepted Research Articles -

  • Burnham, D.N., Tyagi, S.R., Uhlinger, D.J., and Lambeth, J.D. (1989) "Diacylglycerol Generation and Phosphoinositide Turnover in Human Neutrophils: Effects of Particulate versus Soluble Stimuli", Arch. Biochem. Biophys. 269, 345-353.
  • Tyagi, S.R., Burnham, D.N. and Lambeth, J.D. (1989) "On the Biological Occurrence and Regulation of 1-Acyl and 1-O-Alkyl-Diradylylycerols in Human Neutrophils: Selective Destruction of Diacyl Species Using Rhizopus Lipase" J. Biol. Chem. 264, 12977-12982.
  • Lambeth, J.D. and Xu, X.X. (1989) "Cholesterol Sulfate: A Naturally-Occurring Inhibitor of Cholesterol Side-Chain Cleavage Which Functions at the Level of Intramitochondrial Cholesterol Translocation" Endocrine Research 15, 85-99.
  • Xu, X.X. and Lambeth, J.D. (1989) "Cholesterol Sulfate is a Naturally Occurring Inhibitor of Steroidogenesis in Isolated Rat Adrenal Mitochondria" J. Biol. Chem. 264, 7222-7227.
  • Lambeth, J.D. (1989) "Introduction to: Minireview Series on the Respiratory Burst Oxidase and its Regulation", J. Bioenergetics and Biomembranes 20, 633-635.
  • Lambeth, J.D. (1989) "Activation of the Respiratory Burst Oxidase in Neutrophils: On the Role of Membrane-Derived Second Messengers, Ca++ and Protein Kinase C" J. Bioenergetics and Biomembranes 20, 709-733.
  • Merrill, A.H., Jr., Nimkar, S., Menaldino, D., Hannun, Y.A., Loomis, C., Bell, R.M., Tyagi, S.R., Lambeth, J.D., Stevens, V.L., Hunter, R., and Liotta, D.C. (1989) "Structural requirements for long-chain (sphingoid) base inhibition of protein kinase C in vitro and for the cellular effects of these compounds" Biochemistry, 28, 3138-3145.
  • Tamura, M., Tamura, T., Tyagi, S.R., and Lambeth, J.D. (1988) "The Superoxide-Generating Respiratory Burst Oxidase of Human Neutrophil Plasma Membrane: Phosphatidylserine as an Effector of the Activated Enzyme" J. Biol. Chem. 263, 17621-17626.
  • Fowler, W.T.,Jr., Lambeth, J.D., and Powell, G.L. (1988) "Photoreactive Cardiolipin Analogs" Chem. Phys. Lipids 47, 261-271.
  • Nishimoto, Y., Tamura, M., and Lambeth, J.D. (1988) "A Menadione-Stimulated Pyridine Nucleotide Oxidase from Resting Bovine Neutrophil Membranes: Purification, Properties, and Immunochemical Cross-Reactivity with the Human Neutrophil NADPH Oxidase" J. Biol. Chem. 263, 11657-11663.
  • Tyagi, S.R., Tamura, M., Burnham, D. and Lambeth, J.D. (1988) "Phorbol Myristate Acetate Augments Chemoattractant-induced Diglyceride Generation in Human Neutrophils, but Inhibits Phosphoinositide Hydrolysis: Implications for the Mechanism of PMA Priming of the Respiratory Burst" J. Biol. Chem. 263, 13191-13198.
  • Lambeth, J.D., Burnham, D.N. and Tyagi, S.R. (1988) "Sphinganine Effects on Chemoattractant-induced Diacylglycerol Generation, Calcium Fluxes, Superoxide Production, and on Cell Viability in the Human Neutrophil: Delivery of Sphinganine with Bovine Serum Alubumin Minimizes Cytotoxicity without Affecting Inhibition of the Respiratory Burst" J. Biol. Chem. 263, 3818-3822.
  • Deli, E., Kiss, Z., Wilson, E., Lambeth, J.D., and Kuo, J.F. (1987) "Immunocytochemical Localization of Protein Kinase C in Resting and Activated Human Neutrophils" FEBS Lett. 221, 365-369.
  • Lambeth, J.D. (1987) "Cytochrome P-450scc: Specificity and Properties of the Cholesterol Binding Site" Endocrine Research 12, 371-392.
  • Wilson, E., Laster, S.M., Gooding, L.R. and Lambeth, J.D. (1987) "Platelet-derived growth factor stimulates phagocytosis and blocks agonist-induced activation of the neutrophil oxidative burst: A possible cellular mechanism to protect against oxygen radical damage" Proc. Nat. Acad. Sci., U.S.A. 84, 2213-2217.