Rhesus macaque Interleukin-10 (rMamu IL-10)
IL-10 is produced by Th2 cells, certain B cells subsets and LPS activated macrophages. Its effect is mostly regulatory, including inhibiting Th1 responses, antigen presentation and TNF production by monocyte/macrophages, but also supports the viability of mast cells and activared B cells. EBV contains the BCRF1 gene that codes for an IL-10 analog which promotes B cell survival.
rMamu IL-10 cDNA codes for a 178 aa precursor polypeptide including an 18 aa signal sequence (resulting in a 160 aa mature polypeptide) that is 97.8% homologous to human IL-10 (Villinger et al, J Immunol 1995 155:3946-3954)(Genbank accession number L26029).
rMamu IL-10 is produced in the mammalian CHO cell lines transfected with a pED expression vector, followed by affinity purification.
Purity is verified by PAGE electrophrosis and absence of endotoxin by LAL test.
Bioactivity of each lot is tested based on inhibition of IFN- production by ConA stimulated PBMCs. Biological activity levels vary from batch to batch but is generally of the order of 0.5x106 U/mg.