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Rhesus macaque Interleukin-2(rMamu IL-2)

T cell growth factor secreted essentially by T helper cells. IL-2 was originally identified from supernatants of PBMCs stimulated with T cell mitogens. Besides stimulating the expansion and activation of T cells, IL-2 also promotes stimulation and proliferation of thymocytes, B cells and natural killer cells.

Old World nonhuman primate IL-2 cDNA(macaque, cercocebus and baboon) code for a 154 polypeptide (resulting in a 134 aa mature polypeptide) that is 99.9% homologous to human IL-2 (Genbank accession number U19847). One major difference is the presence of an Asp-Thr-Lys motif at position 102-104 instead of Pro-Arg in the human sequence resulting in an extra amino acid residue at this site in Old World primate IL-2 (Villinger et al, J Immunol 1995 155:3946-3954).

rMamu IL-2 is produced as a mature polypeptide in E coli using the pET32 expression vector with an enterokinase digestion site to ensure the absence of extraneous residues at the aminoterminus.

Purity is verified by PAGE electrophoresis and absence of endotoxin by LAL test.

Bioactivity of each lot is tested based on induction of proliferation of the IL-2 dependent HT-2 cell line. Biological activity levels vary from batch to batch but is generally of the order of 4x106 U/mg.

Half-life of rMamu IL-2 in vivo following subcutaneous administration is 3.3 hours.